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《Chinese Journal of Mycology》 2007-03
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Aspergillosis of both ear canals caused by Aspergillus terreus:a case report

LI Fa-zeng1,RAN Yu-ping1,DAI Ya-ling1,PENG Xue-mei2(1.Department of Dermatoverenology,West China Hospital;2.School of Basic and Forensic Medicine,Sichuan University,Chengdu 610041,China)  
Objective A case of aspergillosis of both ear canals of a 55-year-old man was reported.Methods The ear wax taken from both ear canals were examined directly under microscopy, and incubated on Sabouraud dextrose agar (SDA) at 25℃, to identify the morphology and to analysis rDNA domain.Results Under microscopy hyphae were detected from the ear wax. Sand-brown colonies developed after the cerumen taken from both ear canals were incubated on SDA at 25℃.Microscopy and scanning electron microscopy showed:①conidial heads loosely columnar, vesicles subspherical,about 10μm diam.②Conidiogenous cells biseriate, metulae as long as the phialides.③Conidia smooth walled, spherical to broadly ellipsoidal.④Conidiophore stipes smooth walled, hyaline. Nuclear DNA was extracted and the rDNA domain was amplified by PCR. PCR products was sequenced and compared with Gene Bank, which indicated the rDNA domain of the isolated fungus was consistency with Aspergillus terreus ATCC1012.The patient was treated by direct dropping of 4% fluconazol parenteral solution on his both ear canals twice a day and his symptoms completely disappeared after 1 month of treatment.Conclusion A case of aspergillosis of both ear canals was diagnosed and successfully treated by topical application of fluconazol parenteral solution.
【CateGory Index】: R763
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8 TIAN Yi, TANG Xiao-peng, LUO Kai-zhong. Center for Liver Diseases, Second Xiangya Hospital, Central South University, Changsha 410011, China;Nine severe hepatitis patients complicated with lung aspergillosis[J];Chinese Journal of Hepatology;2005-09
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