Study on diagnosis of invasive aspergiuosis by PCR and dot blot hybridization

WANG Li,ZHAI Zhi-fang,HAO Fei,ZHONG Bai-yu(Department of Dermatology,Southwest Hospital of the Third Military Medical University,Chongqing 400038,China)  

Objective To establish and evaluate a molecular diagnostic method for diagnosis of invasive aspergillosis (IA)in animal modal and immunocompromised patients.Methods Rabbit model of IA was established and sera was collected.Fungal DNA was detected in sera from rabbits,health people and immunocompromised patients by PCR combined with species-specific probe hybridization.Results Sixteen in 24 sera samples showed positive for PCR and dot blot hybridization 24 h after innoculation,18/24 after 48 h,19/24 afer 72 h respectively and all showed positive after 96 h.The sensitivity and specificity of the assay were 80% and 95.8%,with 5% positive rate for blood culture.Thirty eight negative sera and 2 false positive sera were found in health people.Sera of 2 IA patients showed positive results.Conclusions PCR combined with species-specific probe hybridization for serum is more sensitive and rapidly than traditional blood culture for early diagnosis of IA in animal model,which may be helpful to monitor the patients at risk for IA.

【Fund】： 重庆市自然科学基金(CSTC:2008BB5291);; 第三军医大学临床科研基金(SWH2007006)
【CateGory Index】： R450;R519

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