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《Journal of Zhejiang University(Medical Sciences)》 2010-05
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Effects of isoflurane,sevoflurane and desflurane on expression of ICAM-1 and VCAM-1 in LPS-induced rat lung microvascular endothelial cells

LUO Xing,MIAO Chang-hong,GE Bao-xue,JIANG Hao(Department of Anesthesiology and Critical Care Medicine,Fudan University,Zhongshan Hospital,Shanghai 200032,China)  
Objective: To investigate the effect of isoflurane,sevoflurane and desflurane on the expression of ICAM-1 and VCAM-1 in LPS-induced rat lung microvascular endothelial cells(RLMVECs).Methods: Cultured LPS-treated RLMVECs were exposed to 0.7,1.0 or 2.0 minimum alveolar concentration(MAC) iosoflurane,sevoflurane or desflurane in 6 h.The protein expression of ICAM-1 and VCAM-1 was determined by Western blot analysis.The expression of ICAM-1 mRNA was detected by reverse-transcription polymerase chain reaction(RT-PCR).Results: Isoflurane at concentration of 1.0 MAC up-regulated the expression of ICAM-1 in LPS-induced RLMVECs(P0.05);while same concentrations of sevoflurane and desflurane down-regulated the expression of ICAM-1(P0.05).Desflurane at concentration of 2.0 MAC up-regulated the expression of ICAM-1 in non-LPS-induced RLMVECs.All the volatile anesthetics down-regulated the expression of VCAM-1 in a dose-dependent manner.Conclusion: Compared to isoflurane,1.0 MAC sevoflurane and desflurane down-regulate the expression of ICAM-1,which may be the molecule mechanism of their protective effect in acute lung injury.
【Fund】: 国家自然科学基金(30872430)
【CateGory Index】: R965
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