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《Zhejiang Medical Journal》 2014-10
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Laminin α4(LAMA4) regulates proliferation and invasion of laryngeal carcinoma Hep-2 cells

WU Zhenhua;LI Qun;GONG Zhaohui;Department of Otorhinolaryngology,the Affiliated Li Huili Hospital,Ningbo University School of Medicine;  
Objective To investigate the effects of laminin a 4(LAMA4) on proliferation and invasion of laryngeal carcinoma Hep-2 cells.Methods Recombinant lentiviral vector with completed LAMA4 gene and LAMA4 siRNA sequences combined with GFP were constructed,respectively.Both of them were transfected into Hep-2 cells.The over-expression and knock-down rates were verified by western blot.MTT and Transwell assays were performed to determine the proliferation and invasion ability of transfected Hep-2 cells.The expressions of MMP-2,MT1-MMP proteins and mRNA were evaluated by Western blot and real-time PCR.Results Recombinant lentiviruses with LAMA4 gene and LAMA4 siRNA were successfully constructed.The virus titer was 5 × 10~5 TU/ μI.Lentivirus-mediated LAMA4 and LAMA4 siRNA were transfected into Hep-2 cells.Lower LAMA4 expression was detected in LAMA4 siRNA group,while over-expression was detected in LAMA4 group as compared to the control group(P0.05).No effect on cell proliferation was detected in both LAMA or LAMA4 siRNA-transfected Hep-2 cells.Compared to control group the invasive ability of Hep-2 cells was increased in LAMA4 over-expression group and reduced in LAMA4 knock-down group(P0.05);meanwhile the expression of MMP-2,MT1-MMP proteins and mRNA levels were increased in LAMA4 over-expression group and decreased in LAMA4 knockdown group(P0.05).Conclusion LAMA4 may be involved in the invasion of laryngeal carcinoma Hep-2 cells by regulating MMP-2 and MT1-MMP expression,suggesting that LAMA4 would be a potential therapeutic target for laryngocarcinoma.
【Fund】: 宁波市科技创新团队项目(2012B82019)
【CateGory Index】: R739.65
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1 WU Zhenhua;LI Qun;GONG Zhaohui;Department of Otorhinolaryngology,the Affiliated Li Huili Hospital,Ningbo University School of Medicine;;Laminin α4(LAMA4) regulates proliferation and invasion of laryngeal carcinoma Hep-2 cells[J];浙江医学;2014-10
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