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《Journal of Jiangsu University(Medicine Edition)》 2008-05
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Effects of ppGalNAcT2 antisense RNA on the biological behacior of human gastric cancer SGC7901 cell line

JIN Mei-fang1,2,SHAO Xue-jun1,WU Shi-liang2(1.The Central Laboratory,the Children′ s Hospital of Soochow University;2.Department of Biochemistry and Molecular Biology,Medical college of Soochow University,Suzhou Jiangsu 215123,China)  
Objective: To study the effect on the cell proliferation and the level of MMP2 and TGF-β1 in the SGC7901 cells caused by antisense blocking pp-GalNAc-T2 gene expressionMethods: The antisense expressing vectors of two different length pp-GalNAc-T2 gene segments were constructed and transfected into SGC7901 cells.A series of subcellular clones aiming at blocking of pp-GalNAc-T2 gene expression of SGC7901 cells were established by G418 selection.After antisense blocking,the cells were first tested with fluorescent microscopy.Then the expression of pp-GalNAc-T2,MMP2 and TGF-β1 gene cells growth were studied with RT-PCR.The effects of ppGalNAcT2 antisense RNA on the proliferation of SGC7901 cells were tested by MTT. Results: The results showed that pp-GalNAc-T2 level in SGC7901 cells was obviously redudced and the cell proliferation was slower after antisense blocking.It indicated that the blocking of pp-GalNAc-T2 gene expression had influence on SGC7901 cell proliferation.It was also revealed that the blocking of pp-GalNAc-T2 gene expression could increase the mRNA and protein level of MMP2 and TGF-β1. Conclusion: The result suggests that pp-GalNAc-T2 has an important role in the cell proliferation,invasion and metastasis of cancer.
【Fund】: 国家自然科学基金资助项目(30670462)
【CateGory Index】: R735.2
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