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《Journal of Jiangsu University(Medicine Edition)》 2013-06
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Effects of postconditioning with sevoflurane on iNOS/NO pathway in rats with acute lung injury induced by lipopolysaccharide

YANG Fen;ZHAO Jian-hua;Department of Anesthesia,Suzhou Municipal Hospital;  
Objective:To investigate the effects of postconditioning with sevoflurane on inducible nitric oxide synthase(iNOS)/nitric oxide(NO) pathway in rats with acute lung injury induced by lipopolysaccharide.Methods:Thirty rats were randomly divided into 5 groups(n = 6),NS group and LPS group,rats received intratracheal instillation of normal saline(1 mL/kg) and lipopolysaccharide(5 mg/kg),respectively;sevoflurane groups(S 0.5,1,2 h),4 h after lipopolysaccharide intratracheal instillation(i.e.after the occurrence of acute lung injury),rats received sevoflurane inhalation(2.4%) for 0.5,1,2 h,respectively,simulating a postconditioning scheme.Lung tissue samples were harvested 6 h after normal saline or lipopolysaccharide intratracheal instillation.Histological examination by light microscopy was performed;iNOS expression and NO concentration in lung parenchyma were measured by immunohistochemistry and with NO Assay Kit.Results:Compared with the NS group,iNOS expression and NO concentration in the LPS group were significantly increased(P 0.01),with more severe lung injury.Compared with the LPS group,iNOS expression and NO concentration in S groups were greatly reduced(P 0.01 or P 0.05),with attenuated lung injury,especially in S1h and S2h groups.Conclusion:Postconditioning with sevoflurane could attenuate lipopolysaccharide-induced acute lung injury in rats through inhibiting iNOS/NO pathway activation and reducing the inflammatory response.
【CateGory Index】: R614
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