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《Journal of University of Science and Technology of China》 2004-03
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Comparative Study on the Interaction Between Two Spectroscopic Probes and Acidic Polysaccharides by Absorption Spectra

GAO Gui-zhen 1,2, JIAO Qing-cai 1, CHEN Lei 1, DING Yi-lei 1 (1. Pharmaceutic Biotechnology Key Laboratory, College of Life Science, Nanjing University, Nanjing 210093, China) ( 2. Department of Biochemistry, Suzhou College, Suzhou 234000, China)  
The absorption spectra of the interaction between two spectroscopic probes (Azur A (AA), Methylene Blue (MB)) and three acidic polysaccharides (Hyaluronic Acid (HA), Chondroitin Sulfate (CS) and Sulfated Pachyman(SP)) were comparatively studied. The characters of the interactions between the two probes and three acidic polysaccharides were compared. The maximum binding numbers of the two probes with three acidic polysaccharides have been obtained, to be 150, 3.37×103, 62 and 73, 54, respectirely. The results showed that AA could interact with both the sulfate group and the carboxylic group of the acidic polysaccharides while MB could only interact with the sulfate group.
【Fund】: 国家科技攻关项目 (No .2 0 0 2EB0 2 0 96 2 )
【CateGory Index】: Q539
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