Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Chinese Journal of Antibiotics》 2019-10
Add to Favorite Get Latest Update

Study on the increase of spinosad yields by protoplast fusion of two kinds of Saccharopolyspora strains

Yi Zhuo;Xia Li-qiu;Ding Xue-zhi;Hu Sheng-biao;Luo Yu-shuang;Wang Hai-long;Tang Ying;Huang Xin;Hunan Provincial Key Laboratory of Microbial Molecular Biology/State Key Laboratory of Freshwater Fish Developmental Biology Jointly Established by the Ministry and the Province, College of Life Science, Hunan Normal University;College of Open Education, Yiyang Radio and Television University;  
Spinosad, produced by Saccharopolyspora spinosa is a new kind of green pesticides with efficacy of chemical pesticides and security of biological ones. This study tries to construct a bacterial strain with high yield of spinosad by protoplast fusion between Saccharopolyspora spinosa and Saccharopolyspora erythraea. A strain which did not produce erythromycins was first constructed and protoplast fusion between it and Saccharopolyspora spinosa was done then. After screening by culture of antibiotic plate medium and HPLC analysis of fermentation broth, four fused strains which could produce spinosyns were acquired. Because the yield of spinosad of all the four fused strains was lower than the original ones, direct screening without antibiotics was done then and a number of fused strains with high-yield spinosad were acquired successfully. Strain B-2, one of fused strains, had the highest production of spinosad with a increase of 331% compared with the original ones. Further confirmation for spinosyns produced by fused strain B-2 were obtained by analysis of mass spectrometry. The production of spinosad of B-2 was stable in the subsequent subculture, which indicated the genetic stability of B-2 was good. This research is a report on enhanced production of spinosad by protoplast fusion between Saccharopolyspora spinosa and Saccharopolyspora erythraea and will provide the important basis for constructing the fused strains with excellent features from Saccharopolyspora spinosa and Saccharopolyspora erythraea.
【Fund】: 国家自然科学基金(No.31070006和No.30870064)
【CateGory Index】: Q93
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 5 Hits
1 Guo Wei-Qun;Zou Qiu-Long;Chen Yuan;Li Neng-Wei;Zhang Xiao-lin;Academy of State Administration of Grain;;Screening and breeding of high spinosad producing strains and preliminary study on metabolism curve of mutation[J];中国抗生素杂志;2014-04
2 ZHANG Qiu-xue1,2 LAN Zhou2 WANG Yang2 WANG Chang-lu1 SONG Yuan3 ZHANG Xiao-lin2(1College of Food Engineering and Biotechnology,Tianjin University of Science and Technology,Tianjin 300457,China)(2Academy of State Administration of Grain,Beijing 100037,China)(3College of Biological Sciences,China Agricultural University,Beijing 100094,China);Study on the Conditions of Electrotransformation in Saccharopolyspora spinosa[J];中国生物工程杂志;2011-05
3 CAI Heng WANG Yan WAN Hong-gui JIANG Dao-hang WANG Han-ling ZHAO Zong-song(College of Biotechnology and Pharmaceutical Engineering,Nanjing University of Technology,Nanjing 210009,China);Research Progress of Spinosad Produced by Saccharopolyspora spinosa[J];中国生物工程杂志;2011-02
4 LUO Li-si1,2,LI Neng-wei2,LI Li2,GUO Wei-qun2,YU Jian-ping1,ZHANG Xiao-lin2(1.College of Life Sciences,Guizhou University,Guiyang 550025;2.Academy of State Administration of Grain,Beijing 100037,China);Studies on High Throughput Screening for High Spinosad-producing Strains with 96-well Plates[J];中国农业科技导报;2010-02
5 HE Yu-ping,DAI Jing-yuan,DAI Peng,HU Xi-zhou,LIN Kai-chun(Wuhan Nature's Favour Bio-engineering Co.,LTD,College of Plant Science and Technology,Huazhong Agricultural University 430070,China);Screening and Breeding of Production Strains of Spinosad[J];中国生物防治;2006-01
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 Jin Weiyue;Song Mingkai;Jiao Wenhao;Jiang Ling;Li Shuang;Xu Xian;State Key Laboratory of Materials-oriented Chemical Engineering,College of Biotechnology and Pharmaceutical Engineering,Nanjing Tech University;State Key Laboratory of Materials-oriented Chemical Engineering,College of Food Science and Light Industry,Nanjing Tech University;;The Screening and Characterization of High-yield-carotenoid Strain from Deinococcus wulumuqiensis R12 by ARTP[J];生物技术通报;2015-12
2 Zhou Yang;Xue Zheng-lian;Qin Yan-fei;Zhao Shi-guang;Liu Yan;Institute of Biologic & Chemical Engineering of Anhui Polytechnic University;Wan Bei Pharmaceutical Co. Ltd.;;Optimization of nosiheptide fermentation condition in 24-well microplates by Streptomyces actuosus[J];中国抗生素杂志;2015-05
3 ZHOU Yang;XUE Zheng-lian;XIA Jun;ZHANG Chong;XING Xin-hui;Institute of Biological and Chemical Engineering of Anhui Polytechnic University;Key Laboratory for Industrial Biocatalysis,Ministry of Education;Institute of Biochemical Engineering,Department of Chemical Engineering,Tsinghua University;;Mutation by using atmospheric and room temperature plasmas and high-throughput screening method for improving production of nosiheptide of Streptomyces actuosu[J];工业微生物;2015-02
4 WU Yang;XU Miao;LUO Lingen;YANG Yan;XIA Liqiu;State Key Laboratory Breeding Base of Microbial Molecular Biology/College of Life Science, Hunan Normal University;;Screening of Butenyl-spinosyn High-yield Strains by Paromomycin Resistance[J];中国生物防治学报;2015-01
5 CHEN Yuan;ZHANG Xiao-Lin;HUANG Ying;LI Xiao-Min;Academy of State Administration of Grain;;Research Progress of Insecticidal Antibiotics[J];农业生物技术学报;2014-11
6 XU Miao;WU Yang;YANG Yan;XIA Li-qiu;College of Life Science, Hunan Normal University/State Key Laboratory of Breeding Base of Microbial Molecular Biology;;Impact on Strain Growth and Spinosad Biosynthesis by Overexpression of Cyclic AMP Receptor Protein Gene in Saccharopolyspora spinosa[J];中国农业科学;2014-18
7 Guo Wei-Qun;Zou Qiu-Long;Chen Yuan;Li Neng-Wei;Zhang Xiao-lin;Academy of State Administration of Grain;;Screening and breeding of high spinosad producing strains and preliminary study on metabolism curve of mutation[J];中国抗生素杂志;2014-04
8 XIA Yanchun;WANG Chao;CHEN Yuan;WU Jianglei;ZOU Qiulong;ZHANG Xiaolin;LI Chun;School of Chemistry and Chemical Engineering/Key Laboratory for Green Process of Chemical Engineering of Xinjiang Bingtuan,Shihezi University;Academy of State Administration of Grain;School of Biology and Pharmaceutical Engineering,Wuhan Polytechnic University;;Enhanced production of spinosad with strains of Saccharopolyspora spinosa by genome shuffling[J];化工学报;2014-09
9 Xia Yanchun;Wang Chao;Wu Jianglei;Zou Qiulong;Li Chun;Zhang Xiaolin;School of Chemistry and Chemical Engineering Key Laboratory for Green Process of Chemical Engineering of Xinjiang Bingtuan Shihezi University;Academy of State Administration of Grain;School of Biology and Pharmaceutical Engineering3Wuhan Polytechnic University;;Extraction and Purification of Spinosad[J];中国粮油学报;2014-03
10 LIU Chun-Hua;ZHANG Qun;WU Nan-Cun;WAN Yao;WU Xiao-Fang;LI Shu-Huai;QIN An-Li;Analysis and Testing Center,CATAS;Hainan Provincial Key Laboratory of Quality and Safety for Tropical Fruits and Vegetables;;Determination of spinosyn A residues in apple by ultra performance liquid chromatography-tandem mass spectrometry[J];食品安全质量检测学报;2014-02
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 MENG You-ting et al(Beijing Radiation Center,Beijing 100015);Mutation Breeding of a Streptomycin avermitlis by ~(60)Co γ-ray Irradiation and Study on Radiation Effect[J];安徽农业科学;2012-16
2 CAI Heng WANG Yan WAN Hong-gui JIANG Dao-hang WANG Han-ling ZHAO Zong-song(College of Biotechnology and Pharmaceutical Engineering,Nanjing University of Technology,Nanjing 210009,China);Research Progress of Spinosad Produced by Saccharopolyspora spinosa[J];中国生物工程杂志;2011-02
3 Cheng Yan1,2,Zhang Kecheng2,Zhao Mingfu1,Shi Yiping2,Sun Lei2(1Plant Protection college of Yunnan Agricultural University,Kunming 650201;2State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,CAAS,Beijing 100193);Research on High-Yield Strain of Wuyiencin By ~(60)Coγ Ray Mutagenesis[J];中国农学通报;2010-02
4 LI Neng-wei1,2,ZHANG Xiao-lin1,GUO Wei-qun1,XIONG Jian2,SONG Wei1,SONG Yuan3(1 Academy of State Administration of Grain,Beijing 100037,China;2 Light Industry and Food College,South China University of Technology,Guangzhou,Guangdong 510640,China;3 College of Biological Sciences,China Agricultural University,Beijing 100094,China);Study on the fermentation media for spinosad production[J];西北农林科技大学学报(自然科学版);2010-01
5 XIONG Jian1,LI Neng-wei1,2,YE Jun1,ZHANG Xiao-lin2(1.College of Light Industry and Food Sciences,South China University of Technology,Guangzhou 51064,China)(2.Academy of State Administration of Grain,beijing 100037,China);Determination of Spinosad with High-Performance Liquid Chromatography[J];现代食品科技;2009-06
6 SONG Wei~(1,2),XIONG Jian~1,GUO Wei-qun~2,ZHANG Xiao-lin~(2*),SONG Yuan~3 (1.College of Light and Food Industry,South China University of Technology,Guangzhou 510640;2.Academy of State Administration of Grain,Beijing 100037;3.College of Biological Sciences,China Agricultural University, Beijing 100193,China);Mutagenic Effects of MNNG Treatment on the Spinosad Producing Strains[J];中国生物防治;2009-02
7 CHEN Ji-hong et al(College of Life Sciences,Hebei University,Baoding,Hebei 071002);Breeding Effect of Space Treatment Breeding on Producting Strains of Spinosad[J];安徽农业科学;2008-12
8 Zhang Guoliang(Zhejiang Grain Scientific Research Institute310002);GREEN GRAIN STORAGE AND PESTS PREVENTION-APPLICATION OF PROTECTANTS[J];粮食储藏;2007-02
9 HU Xu-ye,ZHANG Qin,HU Hai-feng*,ZHU Bao-quan(Shanghai Institute of Pharmaceutical Industry,Shanghai 200040);Research on the Fermentation Conditions of Spinosad[J];中国医药工业杂志;2007-02
10 JIN Zhihua a,b,**, CHENG Xiu b and CEN Peilin b a Department of Biological and Pharmaceutical Engineering, Ningbo Institute of Technology, Zhejiang University, Ningbo 315100, China b Department of Biochemical and Chemical Engineering, Zhejiang University, Hangzhou 310027, China;Effects of Glucose and Phosphate on Spinosad Fermentation by Sac-charopolyspora spinosa[J];Chinese Journal of Chemical Engineering;2006-04
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved