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《CHINESE JOURNAL OF NATIBIOTICS》 1995-01
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Studies on formation ,regeneration and fusion of protoplast in Micromonospora echinospora var.producer of micronomicin

Zhao Min;Xie Fenglong;Wang Jie;Yang Xiaowei and Fan Jin (Jiangsu Institute of Microbiology,Wuxi 214063)  
Sttidies on the formation of protoplast in M. echinospora var. shows that the presence of glycine(gly)could defer the germination of spora and grovvth of mycelia ofM.echinospora var.,the affection degree is dependent on the concentration of gly and the content of mycelium media,given medium Ⅱ 0.3 percent of gly could cause apparent changes in morphology of the mycelia,0.5 percent of gly almost compietly inhibit the germination of spora. Compared with lysozyme,the cofnbination of achromopeptidase andlysozyme could give mtich higher formation ratio of protoplast. The ratio of protoplastregeneration on separation media supplemented with 0.2mol/L sucrose could reach 15percent.The recombination ratio could reach 0.5~1.0 percent when 42%percent of PEG4000 (w /v)was used as promoter of the fusion and the recombinants were identified directly on dual drug plate,Many of the recombinants have different secondary metabolic characters with the mother strains. Some fine regenerators have much higher productivity of micronomicin than their mother strains.
【CateGory Index】: R915
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