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《CHINESE JOURNAL OF ANTIBIOTICS》 1999-04
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Improvement of thienamycin producing strain by regeneration and mutagenesis of protoplasts

Cui Dapeng and Shi Lianying (Institute of Medicinal Biotechnology, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100050)  
Protoplast regeneration combined with physical and chemical mutagenization was performed to improve thienamycin production of Streptomyces cattleya . Under the optimum condition, the concentration and regeneration frequency of protoplast were 1.6×10 9/ml and 29.5% respectively. After protoplast regeneration, the curve of normal distribution of relative titer had a positive mutation and mutant PR 117 was obtained, whose relative titer was 60 percent higher than original strains. Spores and protoplasts of strain PR 117 were treated with UV and NaHSO 3 respectively. Results showed that compared with spores, protoplasts were more sensitive to mutagen, death rate increased and the curve of distribution of relative titer was wider after these treatments. When treated with UV irradiation, the rate of positive mutants of protoplasts and spores were 16.5% and 9% respectively and mutant PRu 336 was obtained, whose relative titer was 90% higher than that of original strain. When treated with NaHSO 3, this positive mutation rate of protoplasts and spores were both 4%.
【Fund】: 国家“九五”攻关项目
【CateGory Index】: R915
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