Icarrin-Induced Apoptosis and Related Mechanisms in Human Cancer Cells

Li Guixin, Zhang Ling, Wang Yun, Mao Haiting, Cui Zhengyan, Li Xiaobing (Department of Immunology , Institute of Basic Medicine, Shandong Academy of Medical Sciences , Jinan 250062 )  

To examine if human acute promyelocytic leukemia cells (HL-60) apoptosis of could be inducedby icarrin(ICA, a mononer purified from Epimedium Koreanum Nakai). Methods: The cells undergoing apoptosis were determined by morphology, DNA gel electrophoresis and flow cytometry (FCM). The expression of apoptosis associated genes bcl-2 and c-myc were evaluated by FCM and semi-quantitative RT-PCR methods. Results: ICA induced apoptosis showed classical morphological and biochemistry features in human cancer cells in a time and dose dependent manner in vitro. While inducing apoptosis, ICA downregulated expression of apoptosis associated genes bcl-2 and c-myc. Conclusion : This study suggests that ICA induced apoptosis of HL-60 cells is associated with downregulation of bcl-2 and c-myc genes rnRNA and protein expression.

【Key Words】： icarrin HL-60 cells apoptosis gene expression
【CateGory Index】： R73-36

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