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《Chinese Journal of Cancer Biotherapy》 2002-02
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Expression and Purification of Recombinant Human Endostatin

LI Zhong-yi, WANG Jun-yuan, LIU Jiang-qiu, CHEN Lin-sheng , LI Ning ( Military Medical Institute of Shenyang Military Rigion, Shenyang 110034, China; Liaoning Tumor Biological Gene-Therapy Center)  
Objective: To highly express rh-Endostatin from Pichia pastoris and purify it to homogeneity. Methods: Constructed Pichia pastoris X33/pLW202 was amplified and inoculated to ferment media. The supernatant of the strain was collected after induction. Through ultrafiltration, affinity and gel chromatography, rh-Endostatin with high purication was obtained. Results: 60mg/L rh-Endostatin was obstained from supernatant. HPLC showed its purity was above 98%. Conclusion: High level expression of secreted rh-Endostatin has been successfully achieved in Pichia pastoris expression system.
【Fund】: 辽宁省科学技术厅(2001226001)基金资助
【CateGory Index】: R73-3
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