Full-Text Search：
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Chinese Journal of Cancer Biotherapy》 2002-04
Add to Favorite Get Latest Update

Effect of Tiam-1 Antisense Oligodeoxynucleotides (ASODNS)on Antimetasta-sis of Tumor

LI Zhao-zhong, ZHANG Ling, MAO Hai-ting, WANG Yun, LI Deng-hua, GUI Shu-ling(Insteitute of Basic Medicine, Shandong Academy of Medical Sciences, Jinan 250062 , China)  
Objective:Tiam-1 antisense gene was used to downregulate Tiam-1 mRNA and protein expression, thus tumor metastasis could be suppressed. Methods; In this study Tiam-1 mRNA was examined by RT-PCR. The expression of Rho protein was examined by flow cytometry. Basement membrane was used to examine the effect of Tiam-1 antisense gene on metastatic behavior of PG cells. Results; The relative expression level of Tiam-1 mRNA was reduced after exposure to Tiam-1 ASODNS-Lf. Only 72 hours of exposure to Tiam-1 ASODNS, cell metastasis of treated group was inhibitted significantly. The invasive cell numbers through matrigel reduced from 945±40 to 649 ±35 ( P 0. 05 ). Tiam-1 ASODNS-Lf significantly inhibitted Rho protein expression ratio from 27. 86% to 16. 86% (P 0. 001) , which related to signal transduction pathway. Conclusions: Antisense gene inhibits invasion and metastasis of tumor cells through a cascade reaction of gene-protein-cell effect and provides the theoretical and experimental bases for clinical therapy and study of antimetastasis.
【Key Words】： antisenes oligodeoxyribonucleotides Tiam-1 gene Rho protein metastasis
【Fund】： 山东省自然科学基金(Y99C06)
【CateGory Index】： R73-36
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 2 Hits
1 FEI Xi-jia,XU De-chang*(School of Food Science and Engineering,Harbin Institute of Technology,Harbin 150090,China);Analysis of miRNAs microarray data and anti-cancer pathway prediction of β-sitosterol[J];China Journal of Bioinformatics;2012-01
2 ZHU Jin-ming, YU Pei-wu, ZHAO Yong-liang(Department of General Surgery, Southwest Hospital, The Third Millitary Medical University, Chongqing 400038, China);Relationship between the expression of Tiam1, Rac1 and the pathobiological behavior of gastric cancer[J];Chinese Journal of General Surgery;2005-03
【Citations】
Chinese Journal Full-text Database 1 Hits
1 Zhang Chongjie, Wei Dapeng, Zhao Zongrong, Liu Jie, Zhang Ping (Dept. of Immunol, West China University of Medical Sciences, Chengdu 610041);The Effect of Pro-inflammatory Stimulators on Lymphocytes Binding to and Penetrating Through Endothelial Cells[J];Shanghai J.of Immunology;1997-02
【Co-citations】
Chinese Journal Full-text Database 4 Hits
1 HAO Yu,QIU Quanying,WU Jun (Department of Immunology,Beijing University of Chinese Medicine,Beijing 100029,China);Effect of astragalus polysaccharin(APS)on lymphocyte-endothelium adhesion and the molecular mechanism[J];IMMUNOLOGICAL JOURNAL;2000-03
2 MAO Hai-Ting, ZHANG Ling, WANG Yun, LI Xiao-Bing (Dept of Immunoloy, Institute of Basic Medical Sciences, Shandong Academy of Medical Sciences, Jinan 250062);Studies of icariin and pseudomonas jinanensis agent on anti-metastatic mechanism in vitro[J];Chinese Pharmacological Bulletin;2000-05
3 MAO HaiTing, ZHANG Ling, WANG Yun et al Institute of Basic Medical Sciences, Shandong Academy of Medical Sciences, Jinan 250062;Studies of suppression on invasion and metastasis ability of high metastatic human lung tumor cell line[J];Chinese Journal of Immunology;2001-01
4 Mao Haiting,Zhang Ling, Wang Yun,Li Xiaobing (Institute of Basic Medical Sciences,Shandong Academy of Medical Sciences, Jinan 250062);Experimental Studies of Icariin on Anticancer Mechanism[J];Journal of Chinese Medicinal Materials;2000-09
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 ZHONG Da-ping, LI Yan, ZHOU Jin-ming, ZHOU Qi, JIANG You-zhao, YANG Qi-hong, LIANG Hou-jie (Department of Oncology, Southwest Hospital, Third military Medical University, Chongqing 400038, China);Silenced Tiam1 gene inhibits the expressions of VEGF-C/-D in colorectal cancer cells[J];Acta Academiae Medicinae Militaris Tertiae;2008-17
2 SUN Qing1, DING Yan-qing1, GAO Xue-qin2, YAN Shi2, HAN Jin-xiang21Department of Pathology, First Military Medical University, Guangzhou 510515, China; 2Biotechnology Center, Shandong Academy of Medical Science, Ji'nan 250062, China;Development and application of cDNA microarray of tumor metastasis-associated genes[J];Journal of First Military Medical University;2002-12
3 LIU Tao,TANG Hua Tianjin Life Science Research Center,Tianjin Medical University,Tianjin,300070,China;Technology of Nucleic Acid Labeling in MicroRNA Microarray[J];Journal of Medical Molecular Biology;2007-05
4 Cheng Yongqiang Li Zhengping Wang Yucong Fan Yongshan(Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of Ministry of Education,College of Chemistry and Environmental Science,Hebei University,Baoding 071002,China);MicroRNA Detection[J];Progress in Chemistry;2010-08
5 MO Ligen~1 WANG Huihe~2 HUANG Guangwu~2 ZHAO Huiliu~1 KUANG Guoqian~1 (~1Department of Head and Neck Surgery,the Affiliated Cancer Hospital of Guangxi Medical University,Nanning,530021,China;~2 Department of Otolaryngology,the First Affiliated Hospital of Guangxi Medical University);Correlation between expression of the Tiam1 gene and the invasion and metastasis in nasopharyngeal carcinoma[J];Journal of Clinical Otorhinolaryngology;2005-17
6 WANG Jianting1 ZHANG Yuxiang2(1Department of Otorhinolaryngology Head and Neck Surgery,Beijing Chaoyang Hospital,Capital Medical University,Beijing,100020,China;2Department of Biochemistry&Molecular Biology,Capital Medical University);Expression of programmed cell death 4 protein is closely correlated with laryngeal squamous cell carcinomas[J];Journal of Clinical Otorhinolaryngology Head and Neck Surgery;2011-12
7 GUO Hua, GAO Jianbo, ZHANG Yonggao,et al. Department of Radiology, The Affiliated No.1 Hospital, Zhengzhou University,Zhengzhou, Henan Province 450052, P. R. China;A Comparatively Study of Spiral CT and Pathology,the Expression of nm23-H_1 in Gastric Carcinoma[J];Journal of Clinical Radiology;2006-10
8 LIU Li~1,XU An-gao~2,WANG Wei~1,ZHANG Qing-ling~1,DING Yan-qing~1(()~1Department of Pathology,Nanfang Hospital,Southern Medical University, Guangzhou 510515,China;()~2Huizhou Central People′s Hospital,Huizhou 516001,China);Expression of Tiam1 in colorectal carcinomas and its clinical significance[J];Chinese Journal of Clinical and Experimental Pathology;2006-02
9 WEI Jin-ting1,LIU Wen-qi2(1.Pharmaceutics Department,Putian University,Putian 351100,China;2.The Affiliated Hospital of Putian University,Putian 351100,China);β-Sitosterol,One of Functional Component in Phytosterols[J];Journal of Putian University;2007-02
10 ZHAO Qian,OU Sui-fang,XIE Xiu-qin.(Department of Pathology,The First Affiliated Hospital of Guangdong College of Pharmacy,Guangzhou 510080,Guangdong,P.R.China);Expression p53 and nm23 and its correlation with lymph node metastasis in human gastric cancer.[J];China Tropical Medicine;2007-05
【Secondary References】
Chinese Journal Full-text Database 9 Hits
1 ZHU Jin-ming, YU Pei-wu, ZHAO Yong-liang Department of General Surgery, Southwest Hospital, Third Millitary Medical University, Chongqing 400038, P.R.China;Correlation between Tiam 1 and malignant biological phenotypes of gastric cancer cells[J];Chinese Journal of Cancer Prevention and Treatment;2008-07
2 ZHU Jin-ming,YU Pei-wu,ZHAO Yong-liang Department of General Surgery,Southwest Hospital,Third Millitary Medical University,Chongqing 400038,P.R.China;Effects of antisense inhibition against Tiam 1 on cytoskeleton of gastric cancer cells and its tumorgenesis or metastasis in nude mice[J];Chinese Journal of Cancer Prevention and Treatment;2008-23
3 ZHANG Shu-ping1,DAI Jia-li2,XU Zhou3.1.the First People′s Hospital of Yibin,Yibin,Sichuan 644000;2.the Affiliated Hospital of Luzhou Medical College,Luzhou,Sichuan 646000;3.Affiliated Chinese Medicine Hospital of Luzhou Medical College,Luzhou,Sichuan 646000,China;Expression of Rac1 and MMP-2 in gastric cancer and effects on tumor invasion and metastasis.[J];Sichuan Medical Journal;2010-09
4 LIU Fu-dan,LV Yan-qing,YE Kai-he,et al(Department of Pharmacology,Pharmacy College of Jinan University,Guangzhou,510632,China);Effect of FR/MA on invasion of HepG2 human hepatocellular liver carcinoma cell line in vitro[J];Journal of Practical Oncology;2009-04
5 SUN Si-jing,ZHANG Jun-hua(1.Department of General Surgery,the First Hospital of Zibo City,Zibo,Shandong 255200;2.the Affiliated Hospital of Liaoning Medical College,Jinzhou,Liaoning 121001);RESEARCH OF EXPRESSION OF Rac1,Cdc42 IN GASTRICC ARCINOMA AND CLINICAL PATHOLOGICAL CHARACTERISTICS OF GASTRIC CANCINOMA[J];China Medical Engineering;2010-01
6 GUAN Bao-jie LIU Li-hua ZHANG En-ying XU Wei Department of Clinical Laboratory,First Hospital of Jilin University,Changchun 130021,China;Quantification of T lymphoma invasion and metastasis inducing factor 1 mRNA and its correlation with gastric cancer biological behaviours[J];Chinese Journal of Laboratory Medicine;2007-09
7 ZHU Jin-ming,YU Pei-wu,ZHAO Yong-liang (Department of General Surgery,Southwest Hospital,the Third Millitary Medical University,Chongqing 400038,China);Correlation of Tiam 1 to invasion and metastasis of gastric cancer cells and to development of tumor xenografts in nude mice[J];Chinese Journal of General Surgery;2007-09
8 XU Wei,ZHANG En-ying,GUAN Bao-jie,et al.(The First Hospital of Jilin University,Changchun 130021,China);Expression and signaling transduction of Tiam1-Rac1 and the study on correlation between it and gastric cancer metastatic[J];Chinese Journal of Laboratory Diagnosis;2007-04
9 ZHANG Jia1,ZHANG Su-qin1,DONG Pin2,HE Jing-chun2,LI Da-wei2(1.Department of Otolaryngology-Head and Neck,Changhai Hospital,the Second Military Medical University,Shanghai 200433,P.R.China;2.Department of Otolaryngology-Head and Neck,Shanghai First People's Hospital,Shanghai Jiaotong University,Shanghai 200080,P.R.China);Expression and significance of Tiam-1,MMP-2 in human laryngeal squamous cell carcinoma[J];China Journal of Modern Medicine;2010-09
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved