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《Chinese Journal of Cancer Biotherapy》 2009-06
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Preparation of tumor-specific CTLs and their therapeutic effects on bone marrow micrometastasis of breast cancer

LIU Yun-jiang*,ZHANG Xiang-mei,YANG Chao(Department of Surgery,Fourth Affiliated Hospital of Hebei Medical University,Shijiazhuang 050011,Hebei,China)  
Objective:To prepare auto-tumor-specific cytotoxicity T lymphocytes(CTLs) of breast cancer patients and to observe their therapeutic effects on bone marrow micrometastasis(BMM) of breast cancer.Methods: BMM in 82 patients with primary breast cancer(stage Ⅰto Ⅲ),who were treated in the Fourth Affiliated Hospital of Hebei Medical University from March to December in 2007(all the patients signed paper of informed consent),was exmined by flow cytometry using CK18 and CK19 as marker.Twenty-three patients with BMM were randomly divided into two groups: 17 patients were treated with tumor-specific CTLs(therapy group),and 6 patients were treated with IL-2(control group).Tumor-specific CTLs were induced in vitro from axillary lymph nodes and peripheral blood of breast cancer patients in therapy group,and were reinfused into the same patient 10-14 days after operation.The therapeutic effects of tumor-specific CTLs on BMM of breast cancer patients were observed.Results: Twenty-three cases(28.05%) in 82 breast cancer patients were BMM positive as detected by FCM.BMM positive rates increased with the increase of clinical TNM stages and histological grades of breast cancer,and decreased with the increase of ER and PR protein expression in cancer tissues.Dendritic cells(DCs) were successfully isolated and induced from the peripheral blood of breast cancer patients.Tumor-specific CTLs were induced by co-culturing lymphocytes from axillarey lymph nodes with auto-tumor antigen-impulsed DCs.Fourteen cases in the therapy group became negative of BMM after treatment with tumor-specific CTLs(14/19,82.35%).Only one case in the control group became negative of BMM after treatment with IL-2(1/6,16.67%,P=0.00028).Conclusion: Tumor-specific CTLs have been successfully prepared and they show a satisfactory therapeutic effect on bone marrow micrometastasis of breast cancer.
【Fund】: 河北省科技厅科技支撑课题资助项目(No.05276101D-66No.08276101D-70);; 河北省高校强势特色学科基金资助项目~~
【CateGory Index】: R737.9
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【Citations】
Chinese Journal Full-text Database 4 Hits
1 LIU Yun-jiang,WU Feng-yun,WU Xiang-de.(Department of Surgery,the Fourth Hospital of Hebei Medical University,Shijiazhuang 050011,China);Detection of micrometastases in bone marrow of patients with breast cancer and its clinical significance[J];Journal of Clinical Surgery;2004-09
2 LIU Yun-jiang~1, ZHANG Jian-li~1, SHAN Bao-en~ 2* ~1Department of Surgery, ~2Research center, the Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, China;Induction of specific CTL by DC from axillary lymph node of patients with breast cancer[J];Chinese Journal of Cellular and Molecular Immunology;2007-01
3 Liu Yunjiang Liu Xianyi Liu WeiDepartment of Surgery, the No.4 Hospital of Hebei Medical University, Shijiazhuang;hMAM Detection in Bone-Marrow Micrometastasis of Patients with Breast Cancer and Its Significance[J];Chinese Journal of Clinical Oncology;2006-10
4 LIU Yun-Jiang,ZHANG Jian-Li,SHAN Bao-En.The Fourth Hospital of Hebei Medical University,Shijiazhuang 050011,China;Study on DC loaded with freeze-thrawing antigen from breast cancer cells to the specific cytotoxicity of CTL in vitro[J];Chinese Journal of Immunology;2007-08
【Co-citations】
Chinese Journal Full-text Database 5 Hits
1 QI Yi-Xin1,HU Jie2,WU Zhong-Lin1,XU Hai-Bo3,LIU Yun-Jiang1(1. Department of General Surgery, the Fourth Affiliated Hospital, Hebei Medical University, Shijiazhuang 050011,China; 2. Department of Immunology, Hebei Medical University, Shijiazhuang 050017,China; 3. Department of Surgery General Introduction, Hebei Medical University, Shijiazhuang 050017,China);Detecting micrometastasis in bone marrow of breast cancer patients by immunomagnetic-bead combined with scanning electron microscope[J];Bulletin of the Academy of Military Medical Sciences;2009-01
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4 WANG Li1,SONG Lan-ying1,LIU Yun-jiang21.Department of Oncology Surgery,People's Hospital of Xingtai,Xingtai 054031,P.R.China2.Department of Surgery,Forth Hospital of Hebei Medical University,Shijiazhuang 050011,P.R.China;Relationship between uPA/PAI-1 and clinical pathology factors in breast cancer tissues[J];Chinese Journal of Cancer Prevention and Treatment;2009-22
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