Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Chinese Journal of Cancer Biotherapy》 2018-03
Add to Favorite Get Latest Update

Effect of ursolic acid combined with gemcitabine on proliferation and apoptosis of pancreatic cancer PANC-1 cells

JIANG Sen;ZHENG Xi;HE Yan;Tina LIAO;ZHANG Kun;Laboratory of Natural Medicinal Chemistry and Green Chemistry, Guangdong University of Technology;Rutgers Cancer Institute of New Jersey, the State University of New Jersey;Wuyi University;  
Objective: To study the effects of ursolic acid cooperated with gemcitabine on proliferation and apoptosis of pancreatic cancer PANC-1 cells. Methods: Human pancreatic cancer cell line PANC-1 was cultured in vitro with ursolic acid and gemcitabine respectively; and MTT assay was used to determine the IC_(50) of ursolic acid and gemcitabine, thus obtaining the best drug concentration. Ursolic acid(2 μmol/L) and gemcitabine(0.2 μmol/L) alone or in combination was used to treat PANC-1 cells; trypan blue assay was used to test cell viability, and PI staining was used to examine the cell apoptosis; wound healing was used to detect the proliferation and migration of PANC-1 cells. The protein expressions of P-JNK, Bcl-2, IL-6, P-Stat 3, NF-κB and Cox-2 in cells of each treatment group were detected using Western blotting. Results: Both ursolic acid and gemcitabine could significantly inhibit the proliferation of PANC-1 cells, and the IC_(50) is 13.67 and 2.78 μmol/L, respectively; and the final concentrations were determined at 2 and 0.2 μmol/L for ursolic acid and gemcitabine, respectively. Compared with single drug treatment, the combined treatment exerted a more prominent cell proliferation inhibition effect([46.47±5.07]% vs [78.38±8.65]%, [76.12±3.23]%, all P0.05), apoptosis-induction effect([39.78±7.01]% vs [20.35 ± 8.51]%, [20.35 ± 8.51]%, all P0.01) and migration inhibition effect(P0.01) on PANC-1 cells. Western blotting showed that the combined treatment strongly inhibited Bcl-2 and IL-6 expression, accelerated P-JNK protein expression compared with single drug treatment. Conclusion: The synergistic effect of ursolic acid and gemcitabine enhanced the inhibition on proliferation, migration, and promoted cell apoptosis of human pancreatic cancer cell line PANC-1, the mechanism may be associated with inhibition of Bcl-2, Il-6, P-stat 3 proteins and promotion of P-JNK protein.
【Fund】: 国家自然科学基金资助项目(No.81272452)~~
【CateGory Index】: R735.9
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved