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《Chinese Journal of Cancer Biotherapy》 2018-03
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The effect of FBXW7 gene point mutation to colorectal cancer HCT-116 cells' proliferation, migration, invasion, and its apoptosis resistance

ZHANG Baichuan;CHENG Yong;WANG Xiangfeng;TANG Kang;WANG Wuyi;Department of Gastrointestinal Surgery,the1st Hospital Affiliated to Chongqing Medical University;Department of General Surgery,Daping Hospital and the Research Institute of Surgery, Third Military Medical University;  
Objective: To investigate the impact of FBXW7 gene mutation on cell proliferation, apoptosis, migration, and invasion processes of colorectal cancer HCT-116 cell line. Methods: Recombinant plasmids carrying wild-type and mutant-type FBXW7 SNP were constructed and transfected into HCT-116 cell line; the FBXW7 protein expression level in HCT-116 strains after transfection was detected by Western blotting. Subsequently, cell proliferation capacity was tested by CCK-8 assay; tumor cell colony formation ability was tested by HTCA; cell apoptosis function was tested by FCM; cell migration and invasion were tested by scratch assay and Transwell assay, respectively. Results: Higher HBXW7 protein expression level was detected in HCT-116 strain transfected with wild-type HBXW7 in comparison to the control group(strains transfected with mutant-type HBXW7), negative-control(strains transfected with empty plasmids), and blank-control(strains untransfected)(all P0.05). Compared with the other groups, strains transfected with wildtype HBXW7 exhibited significantly reduced proliferation, colony formation, migration and invasion ability(all P0.05), but obviously increased apoptosis rate(P0.05). Conclusion:FBXW7 gene mutation can down-regulate its protein expression, and further promote the proliferation, migration and invasion as well as inhibit the apoptosis of HCT-116 cells.
【Fund】: 重庆市科委自然科学基金项目(No.cstc2012jj A10038)~~
【CateGory Index】: R735.3
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