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Study on the scavenging effect on superoxide anion free radical and hydroxyl free radical of cordycep sinensis and mycelium of cultured cordyceps sinensis

ZHANG Xiao-qiang, PU Yue-pu, YIN Li-hong,et al. (Public Health School, Southeast University, Jiangshu,Nanjing 210009)  
Objective To explore the scavenging effects of Cordyceps sinensis and mycelium of cultured Cordyceps sinensis on superoxide anion free radical(SAFR)and hydroxyl free radical( HFR).Methods SAFR was pruduced by 1,2,3-benzentriol auto-oxidation systems and HFR was pruduced by Fenton reaction. The scavenging effects of Cordyceps sinensis and myceliun of cultured Cordyceps sinensis on SAFR and HFR were determined and compared by spectrophotometer.Results All of the Cordycep sinensis,Paecilomyces sinensis,Cephalosporium sinensis and Mortierella siensis could scaveng SAFR and HFR (P0.05), but the scavenging effects on SAFR of these four samples were weaker than those of ascorbic acid and the cleaning out effects on HFR of these four samples were also weaker than those of D-mannitol (P0.05).Conclusions Cordycep sinensis and three kinds of mycelium of cultured Cordycep sinensis all could clean out SAFR and HFR. The effective components of scavenging free radicals in Cordydeps sinensis and myceliun of cultured Cordydeps sinensis were Cordycepin and D-mannitol.SAFR was scavenged by Cordycepin and HFR was cleaned out by both Cordycepin and D-mannitol.
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