The efficacy of epimedium flavonoids on senescence-delaying in drosophila and its underlying molecular mechanisms
SHEN Zi-Yin,YUAN Chun-Yan,HUANG Jian-Hua,et al.Huashan Hospital,Fudan University,Shanghai 200040,China
Objective To investigate senescence-delaying efficacy of epimedium flavonoids (EF) and the underlying mechanisms in model organism drosophila.Methods The effect of EF on longevity of drosophila was detected by lifespan test, and the mechanisms were probed using oligonucleotide microarray.Results Average longevity in control,Vit E group,low- and high-dose EF group was 34.90±23.04,35.25±23.41,41.48±25.00,46.23±26.20 days,respectively. The longevity was increased by high-dose EF by 32.46% (P0.01 or P0.05). The old drosophila displayed up-regulation of respiratory chain related enzymes,proteases,pro-apoptotic genes by 2-fold or more. While enzymes responsible for removal of oxygen free radicals,anti-apoptotic genes,endocrine and immunity related genes were down-regulated by 2-fold or more. After treatment by EF,respiratory chain related enzymes and proteolysis enzymes were decreased and enzymes responsible for removal of oxygen free radicals,cell cycle promoting genes,anti-apoptic genes and immunity related genes were increased.Conclusions Senile drosophila have inherence mechanism of excessive production of free radicals and less decreased removal of it,excessive apoptosis,proteolysis and deficiency of immunity. EF can decrease production and increase removal of free radicals,promote cell proliferation,recover immune function and withstand apoptosis to achieve senescence-delaying efficacy.
【CateGory Index】： R285;