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《Chinese Journal of Gerontology》 2014-02
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Effects of recombinant chemerin protein on visfatin and MCP-1 expression in 3T3-L1 adipocyte

JIAO Yi;CHANG Xi;WANG Li-Feng;Department of Biochemistry,Preclinical Medicine College,Xinjiang Medical University;  
Objective To investigate the effects of recombinant chemerin protein on visfatin and MCP-1 expression in 3T3-L1 adipocyte.Methods After 3T3-L1 preadipocytes were differentiated 10 days,mature adipocytes were treated with different concentration of chemerin(0,25,50,100,200 ng/ml) at various time periods(0,12,24,48 h).The expressions of visfatin and MCP-1 were detected by reverse transcription polymerase chain reaction(RT-PCR).Results Compared with that of control group,100 ng/ml chemerin increased visfation expression after 24 h treatment,at the same time,50,100,200 ng/ml chemerin also increased MCP-1 expression(P0.05).The expressions of visfation and MCP-1 were significantly higher at the dose of 50,100,200 ng/ml of chemerin(P0.05) after 48 h treatment.Conclusions Recombinant chemerin protein could increase the expression of visfatin and MCP-1 in mature 3T3-L1 adiopocytes.
【Fund】: 国家自然科学基金项目(No.30960363);; 新疆科技厅地方病分子生物学实验室开放课题(No.XJDX0208-2009-04)
【CateGory Index】: R587.1
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