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《Chinese Journal of Clinicians(Electronic Edition)》 2015-11
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Study of the anti CD14 monoclonal antibody on NF-kappa B(p65) of alveolar macrophage in rat with acute lung injury

Hou Linyi;ZHANG Ziqi;Jiang Qin;Zhang Jifeng;Lyn Jieping;Zhang Wenkai;Department of Intensive Care Unit,Second Hospital of Shanxi Medical University;The Fifth Middle School of Taiyuan;Department of Anesthesiology,First Hospital of Shanxi Medical University;  
Objective To explore the anti CD14 monoclonal antibody on NF-kappa B(p65) of alveolar macrophage in rat with sepsis acute lung injury. Methods 15 male SD rats(150±20)g were randomly divided into 3 groups, group Sham, group CLP and group Anti-CD14. There were 5 rats in each group. The classic cecal ligation and puncture(CLP) method was used to make the animal model with acute lung injury. After the success of modeling 6 h, we used ELISA to detect plasma MIP-2, IL-10 and Western blot was used to detect(p65) protein expression level in left lung. Inferior lobe of right lung was taken for HE staining and the pathological changes of lung after section observation under light microscope. Sham group as control group. Results ELISA results showed that the plasma MIP-2 levels in group CLP was significantly higher than Sham group and Anti-CD14 group, plasma MIP-2 concentration in Anti-CD14 group was lower than CLP group. There were significant differences(P0.05); compared the plasma concentration of IL-10 in 3 group, there was no statistical difference(P0.05); Western blot results showed that CLP group and the Anti-CD14 group of NF-kappa B(p65) expression quantity was higher than Sham group(P0.05), Anti-CD14 group of NF-kappa B(p65) expression was lower than CLP group(P0.05). HE staining showed that CLP group and Anti-CD14 group lung appeared inflammatory cells, pulmonary interstitial thickening, hemorrhage, pulmonary manifestation in Anti-CD14 group than in CLP group was significantly reduced. Conclusion The anti CD14 monoclonal antibody could bind to receptors on the surface of alveolar macrophages of CD14 specific, prevent lung NF-kappa B(p65) nuclear translocation, reduce inflammatory cytokine production and release and inhibit inflammatory cell infiltration, plays an important role in the lung protective.
【Fund】: 山西省归国留学科研项目(2011-105);; 山西省太原市科技项目(12016905)
【CateGory Index】: R459.7
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