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《Journal of the Chinese Cereals and Oils Association》 2009-04
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Optimizing Anthrone Colorimetric Method in Determination of Legume Starch by Response Surface Methodology & Three-Wavelength Technique

Hao Xiaoyan1 Zhang Jusong2 Gu Weihong3 Ma Hao1(State Key Lab of Crop Genetics and Germplasm Enhancement,Soybean Research Institute,NanJing Agricultural University1,Nanjing 210095)(College of Agronomy,Xinjiang Agricultural University2,Wulumuqi 830052)(Center for Introducing Varieties of Animal and Plant,Shanghai Agricultural Academy3,Shanghai 201106)  
The anthrone colorimetric method combined with threewavelength technique was used to determine the content of starch in legume.Based on the results of single-factor experiments,the response surface methodology was used to establish a quadratic polynomial regression mathematics model on the determination of the starch content by the anthrone colorimetric methed,and then the model was applied to optimize the determining conditions.Results indicate three-wavelength technique is superior to one-wavelength technique.To avoid other carbohydrate besides starch being hydrolyzed,using hydrochloric acid as hydrolytic acid is proper to achieve accurate determination.The optimum factor conditions resulted from the single-factor experiments are hydrochloric acid dose 0.4 mL,hydrolytic time 2 min,anthrone dose 0.5 mL,sulfuric acid dose 5 mL,and color display 3min;the optimum determining conditions resulted from the quadratic polynomial regression mathematics model are anthrone dose 0.5 mL,sulfuric acid dose 4.8 mL, and color diaplay 18.8 min.The established method provides an exact,convenient and fast means to quantify the starch content in legume,meeting the requirement of multi-sample determination.
【Fund】: 新疆维吾尔自治区高校科研计划科学研究重点项目(XJEDU2004I17);; 上海市农科院科技发展基金项目(2000-04-06-3)
【CateGory Index】: S52
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