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《Journal of the Chinese Cereals and Oils Association》 2009-04
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Optimizing Anthrone Colorimetric Method in Determination of Legume Starch by Response Surface Methodology & Three-Wavelength Technique

Hao Xiaoyan1 Zhang Jusong2 Gu Weihong3 Ma Hao1(State Key Lab of Crop Genetics and Germplasm Enhancement,Soybean Research Institute,NanJing Agricultural University1,Nanjing 210095)(College of Agronomy,Xinjiang Agricultural University2,Wulumuqi 830052)(Center for Introducing Varieties of Animal and Plant,Shanghai Agricultural Academy3,Shanghai 201106)  
The anthrone colorimetric method combined with threewavelength technique was used to determine the content of starch in legume.Based on the results of single-factor experiments,the response surface methodology was used to establish a quadratic polynomial regression mathematics model on the determination of the starch content by the anthrone colorimetric methed,and then the model was applied to optimize the determining conditions.Results indicate three-wavelength technique is superior to one-wavelength technique.To avoid other carbohydrate besides starch being hydrolyzed,using hydrochloric acid as hydrolytic acid is proper to achieve accurate determination.The optimum factor conditions resulted from the single-factor experiments are hydrochloric acid dose 0.4 mL,hydrolytic time 2 min,anthrone dose 0.5 mL,sulfuric acid dose 5 mL,and color display 3min;the optimum determining conditions resulted from the quadratic polynomial regression mathematics model are anthrone dose 0.5 mL,sulfuric acid dose 4.8 mL, and color diaplay 18.8 min.The established method provides an exact,convenient and fast means to quantify the starch content in legume,meeting the requirement of multi-sample determination.
【Fund】: 新疆维吾尔自治区高校科研计划科学研究重点项目(XJEDU2004I17);; 上海市农科院科技发展基金项目(2000-04-06-3)
【CateGory Index】: S52
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【Citations】
Chinese Journal Full-text Database 7 Hits
1 Zhao Yongliang (Key Biotech. Lab, Institute of Crops Science, Chinese Agricultural Academy of Science and Technology, Beijing, 100081, China);A Novel Micro-amount Method with Spectrophotometer to Determine Amylose and Total Starch Content of Wheat Seeds[J];Food and Fermentation Industries;2005-08
2 ZHANG Lan-jie,XIN Guang,CHEN Hua,ZHOU Xiao-xuan (Department of Chemistry, Anshan Normal University, Anshan 114007, China);Determination of Total Flavonoids in Qianshan Acanthopanax senticosus Leaves and Fruits by Three-wavelength Spectrophotometry[J];Food Science;2008-03
3 WANG xiping, LI changsheng, et al (Jilin Reclamation and special products professional college,Jilin 132109);Primary discussion on the determination of starch content in food[J];Science and Technology of Cereals,oils and Foods;2001-02
4 Han Zhentai (The Reserch Institute of Forest Ecology,Environment and Protection,Chinese Academy of Forestry,Beijing 100091,China );Determination of Starch Content of the Root of Kudzu Vine By High Performance Liquid Chromatography[J];Modern Scientific Instruments;2004-06
5 CHEN Song,ZHAO Jian,SONG Shao-ling(Sichuan Textile Industry Research Institute,Chengdu 610072,China);Determination of starch content in textiles via perchloric acid[J];Dyeing & Finishing;2006-05
6 Liu PeilingZhang Benshan(College of Light and Food Industry in South China University of Technology,Guangzhou 510640);Non-crystal Starch Granule[J];Journal of the Chinese Cereals and Oils Association;2006-02
7 ZHU Zhi-hua,LI Wei-xi,ZHANG Xiao-fang,LIU Fang,LI Yan,LIU San-cai(The Supervision and Testing Center for Crop Germplasm Resources,MOA/Institute of Crop Sciences,CAAS,Beijing 100081);Evaluation of Protein and Starch Content in Food Legumes Germplasm[J];Journal of Plant Genetic Resources;2005-04
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 Ma Yukun1,Ma Jingfang1*,Huang Haishan1,Guo Yanqing1,Wang Wangtian1,Zhang Xianhu2,Cao Ziyi1,2(1.College of Life Science,Gansu Agricultural University,Lanzhou 730070;2.Postdoctoral Station of Ningxiahong Medlar Industry Ltd.,Zhongwei,Ningxia,755000);Performance of Fujiminori grapevine grafted on different rootstocks in alkaline soil[J];Sino-Overseas Grapevine & Wine;2007-01
2 Huang Haishan1, Ma Jingfang1, Ma Yukun1, Guo Yanqing1, Cao Ziyi1,2,3 (1. College of Life Science, Gansu Agricultural University, Lanzhou 730070; 2. Gansu Province Senior Professors Association Committee on Agriculture, Lanzhou 730070; 3. Ningxia Wolfberry Industry Groups Postdoctoral Workstation, Yinchuan, 750000);Study on the relation between bleeding and cold resistance of grapevine[J];Sino-Overseas Grapevine & Wine;2007-02
3 Li Yonghong~1 Ma Yingmin~(1,2) 1 School of Applied Chemistry and Biological Technology,Shenzhen Polytechnics College,Shenzhen 518000; 2 College of Life Science,South China Agriculture University,Guangzhou,510642;Effects of Drying at Different Rates on Desiccation Sensitivity and Membrane Lipid Peroxidation of Pachira macrocarpa Seeds[J];Chinese Journal of Tropical Crops;2008-06
4 LV Dong-po et al.;The Research Status and Potential Using of IMP[J];Meat Research;2007-11
5 TAN Zeng-quan~1,TIAN Li-ping~1,LIU Qing-guang~2,JIANG Hong~1,LI Yu-qiao~1,ZENG Fan-zhi~1(1 Collge of Life Science,Shihezi University,Shihezi,Xinjiang 832003,China; 2 Library,Shihezi University,Shihezi,Xinjiang 832003,China);The Nutritional Value of Alfalfa Leaf Protein Concentrate in Different Breeding Periods[J];Journal of Shihezi University(Natural Science);2005-05
6 LIU Qing-guang1,ZENG Fan-zhi2,TIAN Li-ping2(1 Teaching Affairs Office of Shihezi University,Shihezi,Xinjiang 832003,China;2 College of Life Sciences,Shihezi University,Shihezi,Xinjiang,832003,China);A Study on the Nutritional Value and Functional Properties of Alfalfa Leaf Protein[J];Journal of Shihezi University(Natural Science);2007-06
7 Chen Jiangping(Taizhou Technical and Vocational College,Taizhou Zhejiang 318020,China);Study on Pectinase Defecation Effect in Seabuckthorns Juice[J];The Global Seabuckthorn Research and Development;2006-02
8 ;几种常见饲料原料中总淀粉含量的测定[J];China Feed;2005-15
9 Fang HuanSheng JipingWu Xianrong;Comparison of papain activity bioassay methods used in industry production[J];Food and Machinery;2000-06
10 ;淀粉晶体结构研究进展[J];Science and Technology of Food Industry;2007-07
China Proceedings of conference Full-text Database 4 Hits
1 ;几种消毒剂对甘蓝种传黑斑病和种子理化特性的影响[A];[C];2006
2 XIONG Yuan-pei (Journal Editing dept.of Xiangfan Branch of the Military College of Economics,Xiangfan 441118,Hubei,China);An Investigation into Quality of Subject Selecting in Optimizing Science Journals[A];[C];2008
3 Chenlongsheng1 2,Pengfangren 2,Chenyongzhong 1 (1.Hunan academy of forestry,Hunan changsha 410004;2.Nanjing Forestry University ,Jiangsu Nanjing 210037);The geographical variance analysis of seed quality of Pistacia chinensis Bunge from different provenances[A];[C];2009
4 HU Qing-su1,2,TAN Xiao-feng1 GONG Bang-chu2*,(1. Central South University of Forest and Technology,Forest college,Changsha 410006;China 2.Research Institute of Subtropical Forestry,CAF,Fuyang 311400,China;);Effects of bagging with different bags on fruit quality of sweet persimmon[A];[C];2009
【Secondary Citations】
Chinese Journal Full-text Database 9 Hits
1 Zhang Benshan et al.;Study on the Crystalline Structure and Property of Corn, Cassava and Potato Starch Granules[J];Food Science;2001-02
2 FENG Bibo, XU Haiyu, CAI Zaisheng(College of Chemistry and Chemical Engineering, Donghua Univer sity, Shanghai 200051, China);Steaming pretreatment with unsaturated steam for cotton fabric with enzymes[J];Dyeing and Finishing;2004-01
3 LI Li;Enzymatic desizing process[J];Dyeing and Finishing;2004-12
4 ZHAO Min1 , WANG Yan2 ,KANG Li1 (1. Forest Resource and environment college, Northeast Forestry University Harbi n, 150040 Heilongjiang China; 2.Applied Chemistry Department, Harbin Institute of Technology, 150001 Heilongji ang China);Study on Activity of Inner Inhibitory Substances of Acantho panax senticosus (Rupr.et Maxim) Harms Fruits and Seeds[J];China Journal of Chinese Materia Medica;2001-08
5 ZHU Zhi-hua, LI Wei-xi, LIU Fang, ZHANG Xiao-fang, WANG Wen-zhen, LIU San-cai, LI Yan (The Supervision and Testing Center for Crop Germplasm Resources,Ministry of Agriculture/Institute of Crop Germplasm Resources, CAAS, Beijing 100081);Identification and Evaluation of Quality Traits in Sorghum Germplasm[J];Journal of Plant Genetic Resources;2003-04
6 LIU San cai, ZHU Zhi hua, ZHANG Jing, ZHANG Xiao fang, LIU Fang, LI Yan, LI Wei xi (Institute of Crop Germplasm Resources, Chinese Academy of Agricultural Sciences, Beijing 100081);Evaluation of Quality Traits between the Two and Six-rowed Barley from American[J];Journal of Plant Genetic Resources;2004-02
7 LI Wei xi, ZHU Zhi hua, LIU San cai, LIU Fang, ZHANG Xiao fang, LI Yan, WANG Shu min (The Supervision and Testing Center for Crop Germplasm Resources, Ministry of Agriculture/ Institute of Crop Germplasm Resources, Chinese Academy of Agricultural Sciences, Beijing 100081);Quality Characters of Chinese Soybean (Glycine max) Varieties and Germplasm Resources[J];Journal of Plant Genetic Resources;2004-02
8 ZHANG Ben shan, ZHANG You quan, ZENG Xin an, YU Shu juan, YNAG Lian sheng (South China University of Technology, Guangzhou 510640,China);INFLUENCE OF DIFFERENT WATER CONTENT ON THE CRYSTALLINE STRUCTURE OF NATIVE CORN STARCH[J];JOURNAL OF ZHENGZHOU GRAIN COLLEGE;2000-02
9 ZHANG Ben shan,ZHANG You quan,ZENG Xin an,YANG Lian sheng (Institute of Light Industry and Chemical Engineering,South China University of Technology,Guangzhou 510640,China);STUDY ON THE SUBMICROCRYSTALLINITY OF PREGELATINIZED CORN STARCH[J];Journal of Zhengzhou Grain College;2000-04
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