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《Chinese Journal of Immunology》 1985-02
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The effects of Ginsenoside on NK activity in citro

Yu yong-Li;Yang Gui-zhen Bethune Medical University  
The paper reported here is about the methodology used in assay of NK activity and the effects of Ginsenoside on NK activity It has been found that the incorporation of ~(125)IUdR into YAC-1 cell line was about 0.6~3cpm/per cell when they were cuculture for 5~6 hrs at 37℃. But the spontaneous release of ~(125)IUdR would increase markedly if the coculture time was over 6 hrs. A satisfied results could be obtained without the treatment of YAC-1 cells with DNA ase and trypsin.The specific cytotoxicity became obvious when effector/target ratio was higher than 100/1.Addition of Ginsenoside in vitro in 10~50μg/ml could enhance NK activity of murine spleen cells while the spontaneous release was similar with the normal control. It was interesting that the spontaneous release would be much higher than the normal control when the Ginsenoside was added at 100μg/ml.
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