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《Chinese Journal of Immunology》 1989-01
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IL-2 mRNA translation in wheat ge(?) extract.

Tian Zhi Gang. Yang Gui Zhen. Department of Immunology.Norman Bethune University of Medical Sciences. Changchun. China.  
Compared with the other two translation systems(rabbit reticulocyte lysate and Ehrlich ascite tumor lysate) in vitro, the wheat germ system was available for IL-2 mRNA translation. We had selected the translation conditions for maximal IL-2 production. in which IL-2 activity was found to be depended on the amount of MLA-144 RNA (0~120μg) or poly (A)~+ RNA (0~5μg) added in the 200μl translating solution. The maximal index of incorporation of protein synthesis was 10.99 and the maximal translation efficiency of IL-2 mRNA was two percentage compared with normal MLA-144 cells.
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【References】
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