Full-Text Search：
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Chinese Journal of Immunology》 2002-03
Add to Favorite Get Latest Update

Study on the inhibition of telomerase activity and regulated mechanism in human cancer cell by Icarrin

ZHANG Ling,WANG Yun,MAO Hai-Ting et al.Institute of Bacic Medicine, Shandong Academy of Medical Sciences,Jinan 250062  
Objective:To study inhibited tecomerase activity of HL-60 cells in human cancer cell by Icarrin(ICA) and its mechanism.Methods:MTT assay,NBT assay, TRAP-PCR,RT-PCR assay,Flow Cytometry.Results:ICA inhibited telomerase activity in HL-60 cells significantly.It was negative correlation between telomerase activity and the expression ratio of CD11b antigen in HL-60 cells.It can induced HL-60 cells to differentiate into mature granulocytes.It can changed cell cycle distribution of HL-60 cells,which was reflected by the accumulation of the vast majority of cells in the G0/G1phase and the loss of cells in the S phase. It can downregulated cell proliferation related gene c-myc;upregulated p21 gene protein and mRNA expression.Conclusion:This study proved mechanism of ICA on inhibited telomerase activity from gene-protein-effect of cell level in HL-60 cells.
【Key Words】： ICA Telomerase activity Cell differentiation Cell cycle Gene expression
【Fund】： 山东省科委资助项目 编号 9712 2 6 2 0 6
【CateGory Index】： R73-36
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 10 Hits
1 ZHENG Lilan,XU Jianqin(College of Veterinary Medicine, China Agricultural University, Beijing, 100094,China);Research Progress on Traditional Chinese Medicine in the Immune Mechanism[J];Progress In Veterinary Medicine;2004-06
2 MENG Ning,KONG Kai,LI Shi-weng(School of Chemical and Biological Engineering,Lanzhou Jiaotong University,Lanzhou 730070,China);Advances in Studies on Chemical Constituents and Pharmaceutical Activities in Species of Epimedium[J];Acta Botanica Boreali-Occidentalia Sinica;2010-05
3 HOU Ji-rui~1, SHENG Ji-min~2, WANG Xiu-quan~1, TIAN Yi-xin~1,　WU Gou-ju~1 (1. College of Chinese Medicinal Material, Jilin Agricultural University, Changchun, Jilin 130118, China; 2. Forestry Bureau of Changbai County, Changbai, Jilin 134400, China );Advances in Studies on Herba epimedii[J];Journal of Jilin Agricultural University;2004-01
4 ZHANG Ming-yue,SHI Jing-xiao (College of Biology and Environmental Sciences,Jishou University,Jishou 416000,Hunan China);Advances in Study of Epimdium L.[J];Journal of Jishou University(Natural Sciences Edition);2009-01
5 WANG Lin-Jiao(School of Vocational Education,Guizhou Normal University,Guiyang,Guizhou 550014,China);Cluster analysis of karyotypies resemblance-near coefficient and evolutionary distance in twenty barrenworts[J];Journal of Guizhou Normal University(Natural Sciences);2008-03
6 CAO Ying-qiang1,MA Si-min2,FAN Xiao-li1,LIN Yuan-xi1,CHEN Ming-xia3,SONG Xin-ai11.Department of Physiology,2.Key Laboratory of Environment and Genes Related to Diseases,Ministry of Education,3.Department of Electron Microscopy,Xi'an Jiaotong University School of Medicine,Xi'an,Shaanxi 710061,China;Epimedium Alleviates Chemotherapy-induced Damage to the Ultrastructure and Function of Rat Epididymides[J];National Journal of Andrology;2008-02
7 SHENG Mao-Yin1,2,CHEN Qing-Fu3(1.Department of Postgraduate,Guizhou Normal University,Baoshanbeilu Road 116,Guiyang 550001,P.R.China;2.School of Life Science,Nanjing University,Hankou Road 80,Nanjing 210093,P.R.China;3.School of Life Science,Guizhou Normal University,Baoshanbeilu Road 116,Guiyang 550001,P.R.China);Studies on crossability of seven species belonging to Epimedium native to Guizhou,China[J];Journal of Guizhou Normal University(Natural Sciences);2010-01
8 Ren Mei-ping Zhong Lin Xiao Shun-han (Department of Pharmacology,Luzhou Medical Colloge, Luzhou, 646000,China);Recent progress in the study of anti-tumor mechanism of traditional Chinese medicines[J];Sichuan Journal of Physiological Sciences;2006-04
9 Qian Wang, Ling Zhang, Hai-Ting Mao, Hong-Tao Gu, Pei-E Wen, Cui-Ling Li, Institute of Basic Medicine, Shan-dong Academy of Medical Sciences, Key Medical Library for Tumor Immunology & Chinese Medicine Immunology of Shandong Province, Key Library for Modern Medicine and Technology of Shandong Province, Jinan 250062, Shandong Province, ChinaShang-Jun Yang, Institute of Materia Medica, Shandong Academy of Medical Sciences, Jinan 250062, Shandong Province, ChinaWu-Qing Xia, Shandong Qianfoshan Hospital, Jinan 250014, Shandong Province, China;Chinese medicine Icraiin reverses expression of hepatocarcinoma cell line HepG2.2.15 and induces differentiation[J];World Chinese Journal of Digestology;2007-19
10 LIU Shao-ying,WU Hong-jin.(Department of Cardioangiology,Beijing Hospital of Integraed Traditional and Western Medicine,Beijing 100039,China);Research Progress of Icariin Pharmacologic Action[J];Medical Recapitulate;2009-06
China Proceedings of conference Full-text Database 2 Hits
1 ZHANG Fengxue, FU Linchun, DENG Wendi, WANG Xinhua. Tropical Medicine Institute,Guangzhou University of Traditional Chinese Medicine,Guangdong 510407,China;Ic-ariin inhibites the apoptosis of CEM x174 triggered by Simian Immunodeficiency Virus infection in vitro[A];[C];2006
2 ZHANG Fengxue,FU Linchun,DENG Wendi,WANG Xinhua~*.Tropical Medicine Institute,Guangzhou University of Traditional Chinese Medicine,Guangdong 510407,China;Ic-ariin inhibites the apoptosis of CEM x174 triggered by Simian Immunodeficiency Virus infection in vitro[A];[C];2006
【Citations】
Chinese Journal Full-text Database 2 Hits
1 ZHAO Yong;ZHANG Ling;CUI Zheng-Yan;LI Shu-Zheng(Department of Immunology, Shandong Academy of Medical Science,Jinan 250001);Effects of icariin on the proliferation and differentiation of HL-60 cells[J];CHINESE PHARMACOLOGICAL BULLETIN;1996-01
2 Zhao Yong;Zhang Ling;Cui Zhengyan;et al(Institute of Basic,Shandong Provincial Academy of Medical Sciences,Jina);Studies on the Immuno Modulatory Action of Icarrin[J];Chinese Traditional and Herbal Drugs;1996-11
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 ZHANG Ying-ying et al (College of Animal Science and Technology,Northwest A & F University, Yangling,Shaanxi 712100);Studies on Screening Compound Diluent Prescriptions with Traditional Chinese Medicine for Bull Semen[J];Journal of Anhui Agricultural Sciences;2007-10
2 DU Wu-ting et al(Key Laboratory of Environmental Science and Biodiversity Conservation of Sichuan Higher Education,College of Life Science,China West Normal University,Nanchong,Sichuan 637002);Comparison of Total Flavonoid Content in Leaves of Different Age Stems of Epimedium sagittatum[J];Journal of Anhui Agricultural Sciences;2009-06
3 Kong Chengfu Third People Hospital of Xiaoshan District,Hangzhou City,Zhejiang Province(311251);Orthogonal Test Optimise the Extract Technical of Yinhuang Granuales[J];Journal of Zhejiang University of Traditional Chinese Medicine;2008-04
4 ZHAO Jun1,2 JIN RiGuang1 LUO Lu1 (1.College of Materials Science and Engineering,Beijing University of Chemical Technology,Beijing 100029;2.National Institute of Pharmaceutical Research & Development Co Ltd,Beijing 102206,China);A correlation between the anti-HIV properties of Chinese medicinal herbs and their trace element content[J];Journal of Beijing University of Chemical Technology(Natural Science Edition);2007-05
5 FU Li-bo1,2,XU Hai-yan2,LIU Feng-lian3,YANG Yan-long1,YANG Yan-ping2(1.Basic Medical College,Jinlin University,Changchun 130021,China;2.College of Life Sciences,Changchun Teachers College,Changchun 130032,China;3.Experiment and Education Technology Center,Linyi Teachers College,Linyi 276005,China);Effects of epimedium on blood pressure in normal and stress-induced rats[J];Journal of Northeast Normal University(Natural Science Edition);2008-01
6 ;益气补肾口服液对体外肿瘤浸润淋巴细胞的作用研究[J];Contemporary Medicine;2009-04
7 Fu Yan-li Xu Zhong-liang Li Yuan-jun (Harbin No.6 Pharmaceutical Factory,Harbin 150056,China);Research on Refining Process of Epimediun Flavonoids by Macroporous Resin[J];Chinese Journal of Medicinal Guide;2008-04
8 SHANG Chuan-chuan1,ZHAO Yi2,MU Yang1,LIANG Ning1,ZHOU Li-mei1,QIU Ming-ning1,WANG Xiao-kun1,LI Nan1(1.College of Veterinary Medicine,Northwest A & F university,Yangling,Shaanxi,712100,China;2.Shaanxi Animal Husbandry & Veterinary Bureau,Xi'an,Shaanxi,710003,China);Effects of Epimedium Polysaccharide on Immune Organs and Immune Effects of Newcastle Disease Vaccine[J];Progress in Veterinary Medicine;2009-04
9 TANG Xiaojun, WANG Yanping, ZHOU Qinghua, ZHU Wen, CHE Guowei, CHEN Xiaohe, ZHU Daxing, SUN Zhilin. Key Laboratory of Lung Cancer Molecular Biology of Sichuan Province, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R.China;Cloning of human telomerase catalytic subunit gene promoter and studying on its specific transcriptional activities in human lung cancer cells[J];Chinese Journal of Lung Cancer;2004-06
10 ZHOU Shu-zhen1,HU Yuan-liang1,ZHANG Bao-kang1,LIU Jia-guo1,WANG De-yun1,WANG Yu-ying2(1.Institute of Traditional Chinese Veterinary Medicine,Nanjing Agricultural University,Nanjing,Jiangsu 210095,China;2.China Agricultural Press,Beijing 100026,China);Effects of different extractive methods on effective component content of new fertility-promoting intrauterine infusion liquid[J];Journal of Fujian Agriculture and Forestry University(Natural Science Edition);2007-03
China Proceedings of conference Full-text Database 2 Hits
1 YU Yan, YAN Hong, HU Senke (Department of Public Health, Xi’an Jiaotong University School of Medicine, No.76, Yanta road West, Xi’an, Shanxi, 710061, China);The Study of the Estrogen-like Effects on Epimedium and Icariin[A];[C];2008
2 Yao Xiaolei,Peng Qinghua.Hunan University of TCM,Changsha,410007,China;The study on the therapeutic effects of extract of buddleja officinalis on dry eye in castrated rabbits[A];[C];2006
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved