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Study on liquid fermentation of Morchella crassipes

LIU Shiwang, MAO Jianwei, WU Yuanfeng, HUANG Jun, YANG Zhixiang, WANG Qiang, YOU Yuru (College of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou 310023, China; Key Laboratory of Chemical & Biological Processing Technology of Farm Products of Zhejiang Province, Hangzhou 310023, China)  
The liquid fermentation conditions of Morchella crassipes were studied. The results showed that the optimum conditions of PYG liquid fer-mentation (0.5% yeast extract) were: fermentation temperature 25℃, rotation speed 150 r/min, medium volume 150 ml in 500 ml flask (adding ten glass beads). It showed that 0.7% CMC addition obviously increased the biomass. Based on above shake flask cultivation conditions, 6 fermentation media were tested, and the highest biomass was obtained on the fermentation medium composed of 50% malt extract, 2% corn powder and 0.5% yeast extract, by which the biomass was 16.4g and the regeneration rate was 9%.
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