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Screening and identification of lactic acid bacteria for high γ-aminobutyric acid production

FAN Jie,SUN Junshe,ZHANG Xiaojie,ZHANG Xiuqing,HU Jinrong,ZHANG Jingsheng(College of Food Science and Nutritional Engineering,China Agricultural University,Beijing 100083,China)  
In this study,using MRS selective medium,six lactic acid bacteria strains producing γ-aminobutyric acid(GABA) were isolated from sauerkraut,pickles,etc.The strain F13,F14 and F15 showed high GABA-producing ability after rescreening.Screening results revealed that the strain F13,F14 and F15 produced 5.01g/L,5.72g/L and 4.98g/L GABA in the fermentation medium containing 10g/L sodium glutamate under 33℃ and for 60h,respectively.According to the morphological characteristics and physiological and biochemical characteristics,the three strains were preliminarily identified as Lactobacillus brevis,Lactobacillus acidophilus,lactobacillus plantarum,respectively.
【Fund】: 国家“863”计划现代食品生物工程技术专题支持课题(2006AA10Z309)
【CateGory Index】: TS201.3
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Chinese Journal Full-text Database 6 Hits
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