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《Journal of Central South University of Forestry & Technology》 2010-12
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Genetic diversity of Pinus massoniana Lamb.seed orchard of Chengbu(Hunan) using ISSR markers

ZHANG Dong-lin1a,2,YANG Yu-jie1a,YANG Mo-hua1a,LI Zhi-hui1a,TANG Xiao-rong3,DING Gui-jie4,TAN Xiao-feng1b(1.Central South University of Forestry and Technology,a.School of Forestry,b.The Key Laboratory of Non-timber Breeding and Cultivation of National Forestry Ministry,Changsha 410004,Hunan,China;2.University of Maine,USA Oromo 04469;3.Hunan Forestry Academy,Changsha 410004,Hunan,China;4.College of Forestry,Guizhou University,Guiyang 550025,Guizhou,China)  
Genetic diversity of 117 clones in 4 populations of Pinus massoniana Lamb.seed orchard in Chengbu,Hunan Province was studied using ISSR markers.Ten ISSR primers,selected from 100 UBC primers,were applied to amplify all clones and a total of 218 markers were obtained.Among them,210 are polymorphic loci and the rate of polymorphic loci is 96.33%.The percentage of polymorphic loci between populations shared a range of 88.99%~90.37% and the highest(90.37%) was between Guangdong and Guangxi populations,which explained its ability adapts to circumstances or environment strongly and the hereditary change level is the highest among the populations.The Shannon diversity index and the Nei's diversity index were 0.503 0 and 0.334 7(why different?).The percentage of genetic identity of the 4 populations ranged from 0.968 9 to 0.981 2 and the coefficient of genetic differentiation and gene flow were 0.039 3 and 12.221 9,respectively.The dendrogram based Nei's genetic distance shows that a high similarity was between Xuefeng and Guizhou populations with the distance of 0.019 0.But there was a lower similarity between Guizhou and Guangdong-Guangxi populations with the distance of 0.031 6.The results reveal their geographic origination,which Xuefeng,Nanling,Guizhou populations are adjacent to each others,while the genetic distance of Guangdong-Guangxi population was farther away from that of the above 3 populations.This result provides a scientific evidence for the management and choosing in Pinus massoniana Lamb.seed orchard.
【Fund】: 教育部博士点基金项目(20060538004);; “十一五”国家科技支撑计划课题(2006BAD24B0301);; 湖南省教育厅重点项目(07A077);湖南省教育厅优秀青年项目(09B112)资助
【CateGory Index】: S791.248
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