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《Chinese Agricultural Science Bulletin》 2005-09
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Applied Study of Bacillus thuringiensis controlling Stored Tobacco Pests

Gao Jiahe(Tobacco Science Research Institute of YNTC, Yuxi Yunnan, 653100)  
Three Bacillus thuringiensis isolates Bt33, Bt53, BtHB, which were separated from tobacco leaves and were high toxic on stored tobacco pests, were systemic studied. The results indicated that: (1) The isolate Bt33 is B.t.subso colmeri and its serotypes is H21., Bt53 isolate is B. t. subso finitimus and its serotypes is H2 but the isolate BtHB is B. t. subso canadensis and its serotypes is H5a5c. (2) The results of interior bioassay toxicity determination showed that after 9 days from inoculation the corrected mortality of Bt33 and Bt53 300 times dilution were over 80% separately on Ephesia elutella Hübner second instar, however that of simply BtHB 600 times dilution could exceed 80%. (3)It was concluded from the stored tobacco leaf controlled effect by Bt33, Bt53, BtHB that the effects of even spraying tobacco leaves by bacterium preparation treatments was better than that of spraying tobacco package surface treatments, and the controlling effect of Bt53 by the former method was best than any other treatments. (4) The chemical component in tobacco leaves was not change for the application of Bacillus thuringiensis preparation; The tasted conclusion could be drawn that the tasting mark of aroma quality, aroma volume, smoke consistence, offensive odor was improved compared to contrast, but there was no distinct difference between the treated leaves and the contrast with anyone of the irratation, physiological strength.
【Fund】: 云南省科技厅科技攻关项目“新型生物制剂控制烟草病虫害研究与应用示范”(2001NG08);; 云南省烟草公司科技项目(01A06)
【CateGory Index】: S435.72
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