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《Chinese Agricultural Science Bulletin》 2005-10
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Construction of Goat Genomic Library

Tang Shuang, An Zhixing, Zhang Mingfeng, Song Hongwei, Zhang Yong (Institute of Bio-engineering, Northwest A & F University, Yangling Shaanxi 712100)  
Goat genomic DNA library was constructed for screening milk-protein-encoding gene of goat and construction of knock-in vector. Genomic DNA was prepared from blood sample, partially digested with Sau3AΙ.15~23kb fragment was fractionated and ligated to Lambda BlueSTAR BamHΙ Arms. Titer of the library is 3.4×106; 1.7×106 clones were obtained with inserts size ranging from 15~23kb.
【Fund】: 国家奶业重大科技专项(2002BA518A01-2)。
【CateGory Index】: S827
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