Full-Text Search:
Home|About CNKI|User Service|中文
Add to Favorite Get Latest Update

Effcets of Insulin-Like Growth Factor 1 on the Proliferation Organic Center Stem Cells at 30 days Growing Period of Antler in Vitro

Feng Haihua1,Zhao Lihong1,Bi Xingming1,Li Guangfeng1,Yue Zhanpeng1,Li Chunyi2,Deng Xuming1(1College of Animal Science and Veterinary Medicine,Jilin University,Changchun 130062;2AgResearch Limited Invermay Agricultural Centre Puddle Alley,Mosgiel,New Zealand,Private Bag 50034)  
OBJECTIVEUsing cell culture techniques to investigate the effcets of insulin-like growth factor 1(IGF1)on the proliferation of antler organic center stem cells(antler stem cells)from various passages at 30 days growing period of antler in vitro;METHODAntler stem cells were isolated from sika deer antler of growing 30 day and cultured.The second,fifth and eighth population cells were incubated with 0,1,3,and 10 nM IGF1.Thymidine incorporation assay was applied after 24 hours of culture respectively to determine the disintegration per minute(DPM)value;RESULTSThe proliferation of cells from growing 30 day's antler in the experiental group(1,3,10nM)was significantly higher than that of the control group(0nM)(p0.01).The average and maximal value of the proliferation of cells in the experiental group was lowest in the second population cells(2987 and 3743 DPM/mg respectively),highest in the fifth population cells(10320 and 12180 DPM/mg),and decreased in the eighth population cells again(8754 and 11568 DPM/mg);CONCLUSIONIGF1 can enhance the proliferation of antler stem cells cultured in vitro.The sensitivity of IGF1 on the antler stem cells from various passages at 30 days growing period of antler in vitro.
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©CNKI All Rights Reserved