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《Chinese Agricultural Science Bulletin》 2008-05
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The Construction of Primary Core Collection of Pomegranate (Punica granatum L.) Germplasm Resources Cultivated in China

Shen Jin1,Zhu Liwu1,Zhang Shuiming1,Gong Xuemei1,Lu Lijuan2(1The key Laboratory of Pomology,Anhui Agricultural University,Hefei 230036; 2Institute of horticulture,Anhui academy of Agricultural sciences,Hefei 230031)  
It is important for preservation,study and application of pomegranate germplarsm resources to establish its primary core collection.Based on 14 botanic and agronomic traits of 135 pomegranate biotypes cultivated in eight origins in China the genetic diversity of total and primary core collection was analyzed by using of Popgen version 1.31 software.Primary core collection was constructed according to systematic and random sample and cluster analysis by SPSS 11.0 for Windows software using squared Euclidean distance coefficient and linkage between groups cluster.The difference of Shannon's information index and Nei's genetic diversity of traits of primary core collection and total collection in different origins were analyzed by T-test,the coincidence rate of traits between primary core collection and total collection was calculated to exam the efficiency of primary core collection constructed.The result showed that the Number of polymorphic loci,Effective locus number,Nei's gene diversity and Shannon's information index was 2~6,1.211~4.084,0.174~0.755 and 0.317~1.511 respectively.The primary core collection was constructed with 41 samples selected from 135 total collections of pomegranate biotypes.There was no positive difference between Nei's genetic diversity of primary core collection and total collection.The average coincidence rate of mean,range,standard deviation and variance coefficient of 14 traits in primary core collection and total collection was 96.77%,95.1%,93.7%,92.1% respectively accounting for the constructed primary core collection could represent genetic diversity of the total collection.
【Fund】: 国家农业综合开发省级示范项目“优质石榴种苗繁育与高产栽培技术示范”(农发组[2001]15);; 安徽省教育厅自然科学基金项目“软籽石榴新品种选育与区域试验示范研究”(2002kj091)
【CateGory Index】: S665.4
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