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《Chinese Agricultural Science Bulletin》 2008-12
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Study on the Detection Methods of Long-term Storage Pollen Viability of Paeonia lactiflora Pall

Li Gang1,Gao Min1,Wang Zibin2,Cheng Shiping1,Shi Jiang1(1College of Agronomy,Luoyang Key Laboratory of Peony Biology,Henan University of Science & Technology,Luoyang 471003 ;2Editorial Department of Journal of Yangzhou University,Jiangsu Yangzhou 225009)  
The viability of Chinese peonies varieties pollen preserved in different temperature(room temperature,4 ℃,-20 ℃)and storage time were detected by I2-KI coloration and TTC coloration,and the germination rate of pollen were detected at the same time by germination in culture medium,to research the correlation of I2-KI,TTC and cultured in vitro.The results were as follow:the pollen viability and germination rate of Chinese peonies varieties reduced gradually as the storage time,and the descent of germination rate was quickest at room temperature;the germination rate of pollen preserved at room temperature for 7 days or 4 ℃ for 6 months reduced to zero,and the germination rate of pollen preserved at-20 ℃ for 9 months reduced to 1.96%.The correlation coefficient between I2-KI,TTC with germination in culture medium,respectively were extremely significant or significant correlate.Compared with TTC,the results of I2-KI in 4 ℃ preserved for 4 months and-20 ℃ preserved for 7 months were more accurate.
【Fund】: 洛阳市科技计划项目“牡丹转基因育种技术研究”(0602042A);; 洛阳市科技计划项目“牡丹新品种选育及产业化开发专项”(0602042A)
【CateGory Index】: S682.12
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【References】
Chinese Journal Full-text Database 4 Hits
1 SHANG Hong-qin(Department of Life Sciences,Heze University,Heze Shandong 274015,China);Effects of Medium Composition on Pollen Germination and Pollen Tube Growth of Paeonia lactiflora[J];Journal of Mountain Agriculture and Biology;2011-04
2 SONG Hong-xia,HOU Lu-fen,WU Zhe,ZHANG Guang-xing (College of Horticulture,Shanxi Agricultural University,Taigu 030801,China);The influence of storage temperature and humidity on carrot pollen viability[J];Acta Agriculturae Shanghai;2011-01
3 Shang Hong-qin (Department of Life Science,Shandong Heze University,Heze 274015,Shandong);Optimization on composition of culture medium for pollen in vitro germination of Paeonia lactiflora[J];Journal of Tibet University(Natural Science Edition);2011-01
4 Wang Qing1,2 Peng Zuodeng1 Guo Qirong2 Feng Yun2 Sun Lifang2(1.Beijing Foresty University,Beijing 100083,China; 2.International Bamboo and Rattan Center,Beijing 100102,China);Pollen Vitality Test and Conservation Methods of Moso Bamboo[J];Journal of Bamboo Research;2012-01
【Citations】
Chinese Journal Full-text Database 6 Hits
1 ZHU Xi-chen1,WANG Jing2,JING Heng2,ZHANG Xiao-feng3,HE Xiao-di2(1.Forestry Station of Wixu Agriculture and Forestry Bureau, Wuxi,Jiangsu 214023,China;2.Horticulture and Plant Conservation College of Yangzhou University,Yangzhou,Jiangsu 225009,China;3.Blue Sky and Green Land Horticulture Nursery,Wuxi,Jiangsu 214023,China);Preliminary study on the determination of Paeonia lactiflora pollen survival ability and the selection of the hybrid parents[J];Journal of Fujian Forestry Science and Technology;2007-02
2 JIANG Xue-ting, DU Yu-hu, ZHANG Shao-ling, WU Jun1(Research Institute of Pomology, Nanjing Agricultural University, Nanjing, Jiangsu 210095 China; Liaoning Agricultural Vocation-Technical College, Yingkou, Liaoning 115009 China);Pollen viability of 43 pear cultivars and comparison of testing methods[J];Journal of Fruit Science;2006-02
3 HONG Yu1, LIU Qiang1, HAN Lan2( 1Department of Biology, Inner Mongolia Normal University, Huhhot 010022, China; 2Department of Biology, Inner Mongolia Normal University, Huhhot 010022, China );Pollen vitality and stigma receptivity of Paeonia lactiflora Pall[J];Guihaia;2003-01
4 Zhang Zixue Sun Feng (Anhui Technical Teachers College 233100);The Selection of the Best Testing Method on the Pollen Viability in Hot Pepper[J];Seed;2002-01
5 ZHAO Hong-bo,CHEN Fa-di,FANG Wei-min(College of Horticulture,Nanjng Agricultural University,Nanjing 210095,Jiangsu,China);Detection methods of pollen viability of Dendranthema[J];Journal of Zhejiang Forestry College;2006-04
6 WANG Qin_Li 2LU Long_Dou 1WU Xiao_Qin 1CHEN Zu_Keng 1LIN Jin_Xing ② 1(Institute of Botany, The Chinese Academy of Sciences, Beijing 100093) 2(Henan Normal University, Xinxiang 453002);Pollen Preservation and Its Viability Test[J];Chinese Bulletin of Botany;2002-03
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 WANG Wen-yuan et al(Shenyang Agricultural University,Shenyang,Liaoning 110161);Effect of Different Temperature and Preserved Condition on the Pollen Viability of Iris[J];Journal of Anhui Agricultural Sciences;2006-23
2 YANG Qin-jun et al (College of Horticulture and Forestry Sciences,Huazhong Agricultural University,Wuhan,Hubei 430070);Study on Pollen Viability of Three Species of Michelia[J];Journal of Anhui Agricultural Sciences;2007-01
3 ZUO Dan-dan et al (College of Horticulture and Landscape Architecture,Yangtze University,Jingzhou,Hubei 434025);Advance in Technique of Plant Pollen Viability[J];Journal of Anhui Agricultural Sciences;2007-16
4 WANG Ding-kang et al (Biological Department,Kunming Teacher's College,Kunming,Yunnan 650031);Studies on Viability Determination of Panax notoginseng Pollen[J];Journal of Anhui Agricultural Sciences;2007-28
5 CHEN Zai-xin et al (College of Horticulture and Gardening, Yangtze University, Jingzhou, Hubei 434025);Research on Pollen Viability of New Chinese Chestnut Lines[J];Journal of Anhui Agricultural Sciences;2008-08
6 LIU Da-sheng et al(Shandong Institute of Environmental Science,Jinan,Shandong 250013);Detection on Pollen Morphology and Viability of Alien Invasive Species Alternanthera philoxeroides[J];Journal of Anhui Agricultural Sciences;2008-35
7 WANG Hua-dong et al(College of Forestry,Gansu Agricultural University,Lanzhou,Gansu 730070);Influence of Low Temperature on the Pollen Viability of Peach Flower[J];Journal of Anhui Agricultural Sciences;2009-11
8 LI Jun-feng et al(South Subtropical Crops Research Institute,Chinese Academy of Tropical Agricultural Sciences,Zhanjiang,Guangdong,524091);Study on Pollen Germination Rate of Agave sisalana[J];Journal of Anhui Agricultural Sciences;2009-34
9 WU Hong-juan et al(College of Landscape Architecture,Beijing Forestry University,National Floriculture Engineering Research Center,Beijing 100083);Research Advances on the Tissue Culture of Peaonia lactiflora Pall.at Home and Abroad[J];Journal of Anhui Agricultural Sciences;2010-02
10 LING Chun-ying et al(Department of Landscape Architecture,Beijing Forestry University,Beijing 100083);Study of Pollen Viability Testing on Cymbidium hybridum and Cymbidium sinense and Their Pollen Preservation[J];Journal of Anhui Agricultural Sciences;2010-05
China Proceedings of conference Full-text Database 10 Hits
1 ZHENG Bao-qiang~1 WANG Yan~1 PENG Zhen-hua~1 LI Li~2 (1 Research Institute of Forestry,Chinese Academy of Forestry,Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration,Beijing 100091;2 College of Horticulture, Agricultural University of Hebei,Baoding 071001);Researches on Pollen Germination and Pollen Preservation Characteristic of Cattleya[A];[C];2011
2 WU Hong-juan~1 YU Xiao-nan~(1,2) SHEN Miao-miao~1 (1 College of Landscape Architechure,Beijing Forestry University,Beijing 100083; 2 National Engineering Research Center for Floriculture,Beijing 100083);Studies on Callus Induction and Differentiation of Herbaceous peony(Paeonia lactiflora)'Fen Yu Nu'[A];[C];2011
3 Liu Debing ,He Ruiai ,Fan Chonghui,Wei Junya and Li Shaopeng ( Horticulture College,South China University of Tropical Agriculture, Danzhou Hainan 571737 ; Northwest Sci-Tech University of Agriculture & Forestry,Yangling Shaanxi 712100j3 Life Science College, Zhongshan University, Guangzhou 510275,China);The Study on Comparison of Determine Chinese Wampee Pollen Activity[A];[C];2004
4 ZHOU Di WANG Xian (Beijing Vegetable Research Center,Beijing Academy of Agricultural and Forest Sciences,Beijing 100097);The Preliminary Research on Identification for the Pollen Viability of 11 Zantedeschia Hybirds Germplasms[A];[C];2007
5 LI Hai-zheng~1 XU Li-cai~(1,2) ZhangFan~1 JIANG Li-gang~1 SHEN Huo-lin~2 (1.National Engineering Research Center for Vegetables,Beijing 100097,China; 2.Vegetable Department of Agriculture and Biotechnology College,China Agriculture University,Beijing 100091,China);Studies on Determination Methods of Pollen Viability and The Optimum pollination time of Summer Squash(Cucurbita pepo L.)[A];[C];2009
6 LIU Yu-yan~1 YU Feng-ming~2 (1 Department of Horticulture and Gardening;2 College of Life Science and Technology, Hebei Normal University of Science and Technology,Changli 066600);Study on Pollen Germination Characteristic of Iris ruthenica var. nana[A];[C];2009
7 Lü Chun-yan WANG Yan ZHU Xiang-tao (Research Institute of Forestry,CAF,SFA Key of Tree Breeding and Cultivation,Beijing 100091);Studies on Comparison of Determination Method about Paeonia lutea’s Pollen Viability[A];[C];2009
8 XING Da-zhou WU Sha-sha DIAO Yi-wei Lü Ying-min (1 College of Landscape Architecture,Beijing Forestry University,Beijing 100083; 2 National Engineering Research Center for Floriculture,Beijing 100083);Studies on the Cross Compatibility within Different Lily Cultivars and Wild Species[A];[C];2009
9 LU Guang-pei YU Xiao-nan REN Jun-wei (Beijing Forestry University,National Engineering Research Center for Floriculture,Beijing 100083);The Construction of Peony Varieties Management Information System[A];[C];2009
10 PAN Tong WU Hong-juan YU Xiao-nan (College of Landscape Architecture,Beijing Forestry University,National Engineering Research Center for Floriculture,Beijing 100083);Primary Study on Multiple Shoots and Callus Induction of Paeonia lactiflora[A];[C];2009
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 Wang Liying et al.(Agricultural College,Shihezi University,Shihezi 832003,China);Study on the Pollen Storage and Viability of Three Lonicera[J];Anhui Agricultural Science Bulletin;2009-07
2 ZUO Dan-dan et al (College of Horticulture and Landscape Architecture,Yangtze University,Jingzhou,Hubei 434025);Advance in Technique of Plant Pollen Viability[J];Journal of Anhui Agricultural Sciences;2007-16
3 WANG Ding-kang et al (Department of Biology,Kunming College,Kunming,Yunnan 650031);Comparison of Determination Methods of Paris polyphylla var.yunnanensis Pollen Activity[J];Journal of Anhui Agricultural Sciences;2007-35
4 WANG Xiao-peng et al (College of Forestry,Shenyang Agricultural University,Shenyang,Liaoning 110161);Screening of the Optimum Method for Determining the Pollen Viability of Viola tricolor Linn[J];Journal of Anhui Agricultural Sciences;2008-07
5 WANG Hong-xia et al (Life Science and Technique Department Xinxiang Medical College,Xinxiang,Henan 453003);Study on Morphology Diversity in Lilium regale Wilson[J];Journal of Anhui Agricultural Sciences;2008-16
6 ZHAI Hong-jie et al (Department of Biology Engineering,Xiangfan Vocational Technical College,Xiangfan,Hubei 441021);Effect of Different Media on Induction,Proliferation and Rooting of Lagerstroemia indica Tube Seedlings[J];Journal of Anhui Agricultural Sciences;2008-21
7 SONG Man-po(Henan Agricultural Vocational College,Zhongmu,Henan 451450);Effect on Cuttage Rooting of Dwarfing Crape Myrtle under Different Concentration of ABT,GGR,NAA[J];Journal of Anhui Agricultural Sciences;2009-27
8 PAN Chun-xiang et al(College of Yingdong Bioengineering,Shaoguan University,Shaoguan,Guangdong 512005);Study of Pollen Viability of Medicinal Dendrobium[J];Journal of Anhui Agricultural Sciences;2009-34
9 WANG Chuang et al (Liaocheng Vocational and Technical College,Liaocheng,Shandong 252000);Research on Tissue Culture and Regeneration Technology of Short Crape Myrtle(Lagerstroemia indica cv. Petite Pinkie)[J];Journal of Anhui Agricultural Sciences;2010-08
10 LI Gui-rong et al(Department of Horticulture,Henan Insititute of Science and Technology,Xinxiang,Henan 453003);The Influence of Boric Acid and Sucrose on Vitality of Lily Pollen[J];Journal of Anhui Agricultural Sciences;2010-18
China Proceedings of conference Full-text Database 2 Hits
1 WANG Jing-zhao~(1,2) WANG Liang-gui~1 YUAN Su-xia~2 LIU Chun~2 MING Jun~2 (1 College of Landscape Gardens,Nanjing Forestry University,Nanjing 210037;2 Institute of Vegetables and Flowers,Chinese Academy of Agricultural Science,Beijing 100081);The Monitoring of Vegetation Resource Based on 3S[A];[C];2011
2 Li Ping, Cheng Fangyun and He Guimei ( Landscape Architecture College,BeiJing Forestry University, Beijing 100083, China);A Primary Study on the Tissue Culture of Paeonbia suffruti-cosa[A];[C];2004
【Secondary References】
Chinese Journal Full-text Database 6 Hits
1 MA Jie;College of Horticulture and Landscape, Henan Institute of Science and Technology;;Effect of Different Storage Temperature and Time on Peach Pollen Viability[J];Journal of Jilin Agricultural Sciences;2013-05
2 ZHANG Yi-ping;WU Li-fang;WANG Xiang-ning;QU Su-ping;CUI Guang-fen;XIA Jing;ZHANG Shi-mei;GUO Shu-hua;WANG Ji-hua;Yunnan Flower Breeding Key Laboratory,Yunnan Flowers Engineering Technology Research Center,Flower Research Institute,Yunnan Academy of Agricultural Sciences;;Research on Pollen Viability and Its Storage Conditions of 32 Lily Genotypes[J];Acta Agriculturae Boreali-Sinica;2013-S1
3 FU Chao;JIA Cai-yun;SONG Hong-xia;College of Horticulture,Shanxi Agriculture University;;Effects of Flowering Stage on Pollen Viability of Chinese Cabbage[J];Heilongjiang Agricultural Sciences;2013-11
4 TANG Li;PANG He;College of Information Technology, Jilin Agricultural University;College of Horticulture, Jilin Agricultural University;;Study on Numerical Taxonomy of Principal Varieties of Cliviaminiata[J];Journal of Jilin Agricultural University;2014-01
5 MA Jie,LI Gui-rong,ZHANG Yu-yuan,LIU Jun-li,LU Xiao-xiao(College of Horticulture and Landscape,Henan Institute of Science and Technology,Xinxiang 453003,China);Influence of Different Conditions on Red Peach Pollen Viability[J];Resource Development & Market;2013-05
6 HUA Xi-qi;ZHOU Wen-wei;JIN Qun-ying;PENG Hua-zheng;SHEN Quan;WANG Kui-hong;HE Qi-jiang;Zhejiang Forestry Academy;Key Laboratory of Bamboo Research of Zhejiang;Changxing Forestry Bureau of Zhejiang;Hangzhou Forestry Academy of Zhejiang;;Flowering and Seeding of Dwarf Phyllostachys praecox and Application of Breeding[J];Journal of Zhejiang Forestry Science and Technology;2013-04
China Proceedings of conference Full-text Database 1 Hits
1 ZHANG Yi-ping WU Li-fang WANG Xiang-ning QU Su-ping CUI Guang-fen XIA Jing ZHANG Shi-mei GUO Shu-hua WANG Ji-hua (Yunnan Flower Breeding Key Laboratory,Yunnan Flowers Engineering Technology Research Center, Flower Research Institute,Yunnan Academy of Agricultural Sciences,Kunming 650205);Research on Pollen Viability and Its Storage Conditions of 32 Lily Genotypes[A];[C];2013
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 Gao Zhimin; Wang Yan; Wang Lianying. Flowers Research and Development Center, CAF, Beijing 100091, P. R. China.;The recent development of research on propagation and breeding of peony.[J];Journal of Beijing Forestry University;2001-04
2 LIU Lin-de~1,ZHANG Ping~1,ZHANG Li~1,CHEN Lei~2,GAO Yu-bao~2 (1 College of Life Science,Yantai Normal University,Yantai,Shandong 264025,China;2 College of Life Sciences,Nankai University,Tianjin 300071,China);Pollen viability,stigma receptivity and pollinators of Weigela florida[J];Acta Botanica Boreali-occidentalia Sinica;2004-08
3 XU Guo-hua~(1,2), WU Hua-qing~1, ZHANG Shao-ling~(1,*) (1 College of Horticulture, Nanjing Agricultural University, Nanjing 210095,China; 2 Department of Biology, Jiangsu College of Education, Nanjing 210013,China);Study on identification of S-genotypes of Chinese pear cultivars[J];Acta Botanica Boreali-occidentalia Sinica;2004-10
4 JIANG Fu-ying 1,2,LI Yan 1,WENG Bo-qi 2 (1.College of Resources and Environment Sciences,Fujian Agriculture and Forestry University,Fuzhou, Fujian 350002,China; 2.Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350003,China);Review on physiology of chilling stress and chilling resistance of plants[J];Fujian Journal of Agricultural Sciences;2002-03
5 XUE Miao nan, LIU Hua ying, RAO Gui rong, YANG Ji hua ( Department of biology, Guangxi Normal University , Guilin 541004, China );Study on low temperature preservation of shatinyu's pollen[J];Guihaia;2000-04
6 HONG Yu1, LIU Qiang1, HAN Lan2( 1Department of Biology, Inner Mongolia Normal University, Huhhot 010022, China; 2Department of Biology, Inner Mongolia Normal University, Huhhot 010022, China );Pollen vitality and stigma receptivity of Paeonia lactiflora Pall[J];Guihaia;2003-01
7 Chen Jiezhong; Xu Chunxiang ;Liang Lifeng(Dept. of Horticulture, South China Agric. Univ., Cuangzhou, 510642);Effect of Low Temperature on Protein and Proline in Banana (Musa spp.) Leaves[J];JOURNAL OF SOUTH CHINA AGRICULTURAL UNIVERSITY;1999-03
8 YU Xiao-yinga,b,LU Xiang-yangb,GONG Ming-fua,XIE Zhi-juna,CAO Huia,DING Jiang-nana (a.College of Horticulture and Landscape;b.Lab of Biochemistry and Fermentation Engineering,HNAU,Changsha 410128,China);Pollen Viability of Senecio hybridus[J];Journal of Hunan Agricultural University;2005-01
9 XueDayu; Ma Yanqing; Huang yanwu(Hunan Vegetable Research Institute,Changsha,410125);EFFECTS OF LOW-TEMPERATURE-STRESS ON ASCORBIC ACID CONTENT OF PEPPER SEEDLINGS[J];JOURNAL OF HUNAN AGRICULTURAL UNIVERSITY;1996-02
10 Wang Shaoxian (Luoyang Agricultural College,Luoyang 471003);Effect of Four Testing Methods to Detect the Pollen Viability of Hot Pepper[J];JOURNAL OF HENAN AGRICULTURAL SCIENCES;1998-12
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