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《Chinese Agricultural Science Bulletin》 2009-05
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Extraction Method Optimizing of Total DNA in Rumen Microorganism of Laoshan Dairy

Sui Changsheng,Wang Lihua(Qingdao Agricultural University,Animal Science and Technology College,Qingdao Shandong 266109)  
The present experiment was conducted to assess DNA extraction method of freezing-thawing and methods combined freezing-thawing and CATB.Results showed that the methods combination of freezing-thawing and CATB could smash cell wall of varieties of micro-organisms including bacteria and Fungus.DNA extraction was stable by this technique and DNA fragments were large enough to be applied in follow-up research.It could be concluded that freezing-thawing and CATB methods combination could extract the total rumen microbial DNA.
【Fund】: 校内基金“高产奶牛TMR生产和饲喂技术的研究(项目编号610634)”;; 山东省自然基金“瘤胃真菌的体外驯化及其分子生物技术测定(项目编号Y2007D73)”资助
【CateGory Index】: S827
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【References】
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【Co-references】
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1 WANG Meng-zhi1,WANG Hong-rong1*,CHEN Ming2,HAO Qing1 (1.College of Animal Science and Technology,Yangzhou University,Yangzhou 225009,China;2.Jiangsu Polytechnic College of Agriculture and Forestry,Zhengjiang Jiangsu 212400,China);The Review on the Application of rDNA Molecular Biology Techniques in Rumen Microbes[J];Grass-Feeding Livestock;2007-01
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4 CHEN Xu-Yu1,ZHOU Ya-kui1,ZHENG Fu-Cong1,2(1.College of Environment and Plant Protection,Hainan University,Danzhou 573717,China;2.Environment and Plant Protection Institute,Chinese Academy of Tropical Agricultuural Science,Danzhou 573717,China);Advance in establishing soil metagenome library with metagenome technology[J];Guangdong Agricultural Sciences;2008-01
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1 TIAN Xiao-juan,ZHANG Zhi-hong,HUANG Jiang-li,HUANG Huang, ZHANG Guo-hua,WANG Dong-sheng,DING Jian-nan(Institute of Biological Resource of Jiangxi Academy of Sciences,Jiangxi Nanchang 330029 PRC);Extraction and Purification of Total DNA from Rumen Microbes in Vitro Fermentation[J];Jiangxi Science;2012-01
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