Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Chinese Agricultural Science Bulletin》 2009-05
Add to Favorite Get Latest Update

Extraction Method Optimizing of Total DNA in Rumen Microorganism of Laoshan Dairy

Sui Changsheng,Wang Lihua(Qingdao Agricultural University,Animal Science and Technology College,Qingdao Shandong 266109)  
The present experiment was conducted to assess DNA extraction method of freezing-thawing and methods combined freezing-thawing and CATB.Results showed that the methods combination of freezing-thawing and CATB could smash cell wall of varieties of micro-organisms including bacteria and Fungus.DNA extraction was stable by this technique and DNA fragments were large enough to be applied in follow-up research.It could be concluded that freezing-thawing and CATB methods combination could extract the total rumen microbial DNA.
【Fund】: 校内基金“高产奶牛TMR生产和饲喂技术的研究(项目编号610634)”;; 山东省自然基金“瘤胃真菌的体外驯化及其分子生物技术测定(项目编号Y2007D73)”资助
【CateGory Index】: S827
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 3 Hits
1 XU Wen-hua 1,HAN Jin-tao 2,CAI Tao 3,XIN Xiao-ling 4,BAI Yan-qin 5,LIU Chao 6,XIN Ya-ping7(1.Niujialiang Animal Husbandry and Veterinary Station in Yuyang,Yulin,Shaanxi 719000;2.Jiangpan Town-level Government in Zhouqu County,Zhouqu,Gansu746300;3.Weinan Animal Husbandry Technology Promotion Center,Weinan,Shaanxi 714300;4.Fuping County Animal Husbandry and Veterinary Station,Fuping,Shaanxi 711700;5.Shijiaban Animal Husbandry and Veterinary Station of Zichang Animal Husbandry and Veterinary Bureau,Zichang,Shaanxi 717300;6.Luochuan Animal Husbandry Bureau,Luochuan,Shaanxi 727400;7.College of Animal Science and Technology,Northwest A&F University,Yangling,Shaanxi 712100);Comparison Test of DNA Extraction Methods from Rumen Microbe of Qinchuan Cattle[J];China Cattle Science;2012-06
2 ZHANG Ling1,HUO Xiang-dong2,LUO Ming1(1.College of Agronomy,Xinjiang Agtricultural University,Urumqi 830052,China;2.Institute of Microbiology,Xinjiang Academy of Agricultural Sciences,Urumqi 830091,China);Extraction and Purification of DNA from Rumen Microbes in Bactrian Camel[J];Xinjiang Agricultural Sciences;2010-04
3 XU Fa-zhi1,WU Sheng-guo2,LI Lv-mu1,DING Xiao-ling1 (1.College of Animal Science and Technology,Anhui Agricultural University,Hefei 230036,China;2.Anhui Anfengtang Animal Medicine Industry Co.,Ltd,Hefei 230036,China);Establishment of a method for extracting goat rumen microbial DNA used in direct PCR amplification[J];Chinese Journal of Microecology;2012-01
Chinese Journal Full-text Database 10 Hits
1 WANG Meng-zhi1,WANG Hong-rong1*,CHEN Ming2,HAO Qing1 (1.College of Animal Science and Technology,Yangzhou University,Yangzhou 225009,China;2.Jiangsu Polytechnic College of Agriculture and Forestry,Zhengjiang Jiangsu 212400,China);The Review on the Application of rDNA Molecular Biology Techniques in Rumen Microbes[J];Grass-Feeding Livestock;2007-01
2 CHENG Yu-mei,DENG Wei-dong,MAO Hua-ming(Faculty of Animal Science and Technologe,Yunnan Agricultural University,Kunming,Yunnan,650201,China);Research Progress on Traditional Techniques and Molecular Biological Techniques in Modern Times for Rumen Microbial Diversity[J];Progress in Veterinary Medicine;2006-S1
3 ZHU Guo-feng, WU Lan, LI Si-guang(School of Life Science, Nanchang University, Nanchang330031, China);Application of RFLP Technology in Microbial Diversity of Lake[J];Environmental Science & Technology;2008-11
4 CHEN Xu-Yu1,ZHOU Ya-kui1,ZHENG Fu-Cong1,2(1.College of Environment and Plant Protection,Hainan University,Danzhou 573717,China;2.Environment and Plant Protection Institute,Chinese Academy of Tropical Agricultuural Science,Danzhou 573717,China);Advance in establishing soil metagenome library with metagenome technology[J];Guangdong Agricultural Sciences;2008-01
5 YANG Shu-li,WANG Jia-qi,BU Deng-pan(State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy ofAgricultural Science, Beijing 100094, China);Study on Quantification Methods of Rumen Microbes[J];China Animal Husbandry & Veterinary Medicine;2007-03
6 LIU Kai-lang,WANG Jia-qi,BU Deng-pan,LI Dan,YU Ping,ZHAO Sheng-guo,XI Xiao-qi(State Key Laboratory of Animal Nutrition,Institute of Animal Science,Chinese Academy of Agricultural Sciences,Beijing 100193,China);Annual Review of Ruminant Nutrition in 2007~2008 Ⅰ.Diversity and Functionality of Rumen Microflora[J];China Animal Husbandry & Veterinary Medicine;2009-01
7 DAN Rui-fang~1,LONG Rui-jun~(1,2*),YANG Yu-hai~3(1.College of Grassland Science,Gansu Agricultural University,Lanzhou,730070;2.Northwest Institute of Plateau Biology,the Chinese Academy of Sciences,Xining,810008;3.Sanjiaocheng Sheep Breeding Farm of Qinghai Province,Gangcha,Qinghai 812300,China,);Improved Method for Extractiog DNA from Microorganisms in Rumen[J];Acta Ecologiae Animalis Domastici;2006-01
8 ZHU Ya-xin,DONG Xiu-zhu,HUANG Li,DONG Zhi-Yang(National Key Laboratory for Pre-Development of Micro-Organism Resources,Institute of Microbiology,Chinese Academy of Sciences,Beijing 100080,China);The Application of BAC Library in Metagenomics Research of Rumen Microbe[J];Journal of Agricultural Science and Technology;2007-05
9 GUO Xue-feng1,LI Hua-wei1,LI Chang-qing2,JIN Hai2,LU De-xun2,Osamu Eni-shi3,Mitsunori Kurihara3(1.College of Animal Science and Animal Medicine Inner Mongolia Agriculture University,Huhhot 010018,Neimenggu,China 2.Inner Mongolia Academy of Agriculture and Animal Science,Huhhot 010030,Neimenggu,China3.National Institute of Livestock and Grassland Science,Tsukuba,Ibaraki 305-0901,Japan);RFLP Analysis of mcrA Gene from Rumen Liquid of Inner Mongolian Cashmere Goats on Different Grazing Stages[J];Journal of Shanxi Normal University(Natural Science Edition);2008-03
10 YAN Bing~(1,2) HONG Kui~1 XU Yun~1 MA Chao~1 [KH*2D](Institute of Tropical Biological Sciences, Chinese Academy of Tropical Agricultural Science,State Key Lab for Tropical Crops Biotechnology, Haikou 571101)~1(Guangxi Mangrove Research Center, Beihai 536000)~2;Metagenome Cloning—A New Approach for Novel Microbial Bioactive Compounds Discovery[J];Microbiology;2005-01
【Secondary References】
Chinese Journal Full-text Database 1 Hits
1 TIAN Xiao-juan,ZHANG Zhi-hong,HUANG Jiang-li,HUANG Huang, ZHANG Guo-hua,WANG Dong-sheng,DING Jian-nan(Institute of Biological Resource of Jiangxi Academy of Sciences,Jiangxi Nanchang 330029 PRC);Extraction and Purification of Total DNA from Rumen Microbes in Vitro Fermentation[J];Jiangxi Science;2012-01
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved