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《Chinese Agricultural Science Bulletin》 2009-05
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Study on Tissue Culture and Leaf Regeneration of Two Kinds of Different Provenances Siberian elm

Wang Jinghua1,Hou Jiansheng2,Liu Guilin1,Liang Haiyong3,Yu Xiaoqing4(1College of Landscape Architecture and Tourism,Agricultural University of Hebei,Baoding Hebei 071001;2Landscape Department,Hebei professional College of Political Science and Law,Shijiazhuang 050061;3College of Forestry,Agricultural University of Hebei,Baoding Heibei 071001;4College of Horticulture,Agricultural University of Hebei,Baoding Hebei 071001)  
Tissue cultures of two stems of Siberian elms,being as explants and from Cangzhou,Hebei and Chifeng,Inner Mongolia respectively,were established in mediums with different concentration.The study shows that the differentiation medium of MS+6-BA 0.10 mg/L+IBA0.005 mg/L is most favorable to the elm from Cangzhou and the differentiation medium of MS+6-BA 0.10 mg/L+IBA0.025 mg/L,to the one from Chifeng,while the rooting medium of MS+IBA0.01 mg/L is most favorable to the elm from Cangzhou and the rooting medium of MS+IBA0.05 mg/L,to the one from Chifeng.MS medium supplemented with 0.005 mg/L TDZ and 0.005 mg/L IAA is the optimal condition for the leaf regenerations of these two Siberian elms.
【CateGory Index】: S792.19
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