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《Chinese Agricultural Science Bulletin》 2009-21
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Study on the proliferation profile of PRRSV in Marc-145 Cells Using Fluorescence Quantitative PCR

Hu Hui1,2, Gao Xiaoping2, Meng Hanbing1, Wang Yabin2, Chen Liying1, Zhang Hongying1, Xin Yinchuan2, Cui Bao'an2 (1College of Animal Science and Veterinary Medicine,Henan Argricultural University, Zhengzhou 450002; 2Henan Key Laboratory for Animal Food Safety, Zhengzhou 450002)  
Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) could grow in the cell line of Marc-145, but the characterization on proliferation is still clearly unknown. This experiment was investigated the replication disciplinarian of PRRSV in the line of Marc-145 using Fluorescence Quantitative PCR. The results showed that the virus began to replicate obviously at the 12 h after inoculation, and the viral proliferation was increased in a great speed between 12 h and 36 h after inoculation. The Marc-145 cells began to release virus at the 18 h after inoculation, and reached the peak at 48 h. This study provides basic information for investigating the pathogenic mechanism and vaccine production of PRRSV.
【Fund】: 河南省杰出人才创新基金项目“猪繁殖障碍性疾病PCR诊断方法建立及标准制订”(0621002100);; 国家质检局项目“猪繁殖与呼吸综合征病毒分子基因库的建立与流行预警机制的研究”(2009IK029)
【CateGory Index】: S852.65
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