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《Chinese Agricultural Science Bulletin》 2011-01
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The Comparison of Different Methods for Isolating Total RNA from Peanuts

Chen Gao 1,2 , Shan Lei 2 , Zhou Lixia 1,2 , Tang Guiying 2 , Bi Yuping 1,2 ( 1 Science College, Shandong Normal Unveristy, Jinan 250014; 2 High-Tech Research Center, Shandong Academy of Agricultural Science, Key Laboratory for Genetic Improvement of Crop, Animal and Poultry of Shandong Province, Key Laboratory of Crop Genetic Improvement and Biotechnology, Huanghuaihai, Ministry of Agriculture, Jinan 250100)  
In order to obtain better quality total RNA in different parts of peanuts, two RNA extraction methods (CTAB and Trizol) and two RNA extraction kits (AXYGEN and TIANDZ) were compared. The results showed that the improved CTAB method was better than Trizol method and two total RNA extraction kits. The total RNA isolated with the improved CTAB method had high integrity, high yield, good stability and no DNA pollution, and then could be used for semi-quantitative RT-PCR and so on.
【Fund】: 山东省自然科学基金项目“花生酰基载体蛋白基因的克隆与功能分析”(ZR2009DM013);; 国家自然科学基金项目“花生二酰甘油酰基转移酶DGAT基因多态性及其对种子油份含量和油份品质的影响”(30871541)
【CateGory Index】: S565.2
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2 RONG Jing;RUAN Meng-bin;WANG Bin;PENG Ming;College of Agriculture,Hainan University;Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences;Key Laboratory of Biology and Genetic Resources of Tropical Crops,Ministry of Agriculture;;Preliminary analysis on genetic characteristics and the promoter activity of MeMYB63 in cassava(Manihot esculenta Crantz)[J];广东农业科学;2014-17
3 Wen Suichao;Luo Jun;Wang Yinquan;Li Shunan;Gansu University of Traditional Chinese Medicine;Key Laboratory of Chemistry and Quality for Traditional Chinese Medicines of the College of Gansu Province;;An Appropriate Method of Isolating Total RNA from Angelica sinensis for Quantitative RT-PCR[J];中国农学通报;2014-21
4 LI Miao;LI Guo-qi;Key Lab for Restoration and Recovery of Degraded Ecosystem in Northwestern of Ministry of Education,Ningxia University;Key Lab of Agricultural Biotechnology of Ningxia;;Comparative Research of Total RNA Extraction Methods FromApocynum venetumL.[J];北方园艺;2014-11
5 ZHANG Xiao-li;DAI Hong-jun;Agricultural College,Ningxia University;;Research Progress on Extraction Method of Plant RNA[J];北方园艺;2014-08
6 WANG Qi-hai;WANG Yi-na;LIU Xiao-hua;WU Wen-jiang;DING Ning;NIU Hong-bin;WANG Lin-zhong;WU Guo-liang;College of Horticulture,Henan Agricultural University;College of Food Science and Technology,Henan Agricultural University;National Center of Engineering and Technological Research for Wheat,Henan Agricultural University;;Comparative Analysis of Total RNA Extraction Methods for Leaves of Peach at Different Development Stages[J];河南农业科学;2014-03
7 SHAN Lei;TANG Gui-Ying;XU Ping-Li;ZHAO Xue-Bin;LIU Zhan-Ji;Bio-Tech Research Centre, Shandong Academy of Agricultural Sciences/Shandong Provincial Key Laboratory of Crop Genetic Improvement,Ecology and Physiology;College of Agriculture,Shandong University;College of Life Science,Shandong Normal University;;Cloning and Analysis of 5′ Flanking Regions of Arachisis hypogaea L. Genes Encoding Plastidial Acyl Carrier Protein[J];作物学报;2014-03
8 TANG Guiying1,LIU Zhanji1,XU Pingli1,ZHAO Xuebin1,2,SHAN Lei1(1 High-Tech Research Center,Shandong Academy of Agricultural Sciences,Shandong Provincial Key Laboratory of Genetic Improvement,Ecology and Physiology,Key Laboratory of Crop Genetic Improvement and Biotechnology,Huanghuaihai,Ministry of Agriculture,Ji’nan 250100,China;2 College of Life Science,Shandong Normal University,Ji’nan 250014,China);Cloning and Functional Analysis of Diacylglycerol Acyltransferase Gene in Arachis hypogaea[J];西北植物学报;2013-05
9 YUAN Hong-mei1,2,ZHAO Li-juan3,GUO Wen-dong4,WU Jian-zhong2,ZHANG Li-guo2,GUAN Feng-zhi2(1.State Key Laboratory of Tree Genetics and Breeding,Northeast Forestry University,Harbin,Heilongjiang 150040;2.Institute of Industrial Crops,Heilongjiang Academy of Agricultural Sciences,Harbin,Heilongjiang 150086;3.Institute of Crop Breeding,Heilongjiang Academy of Agricultural Sciences,Harbin,Heilongjiang 150086;4.Institute of Nature and Ecology,Heilongjiang Academy of Sciences,Harbin,Heilongjiang 150040);Comparison and Analysis of Total RNA Extraction Methods from Populus simonii×P.nigra Pollen[J];北方园艺;2013-06
10 ZHAO Yang1,2,DUAN Cheng-li1(1.School of Forestry and Biotechnology,Zhejiang Agriculture and Forestry University,Zhejiang Linan 311300,China;2.School of Agriculture and Biotechnology,Yunnan Agricultural University,Yunnan Kunming 650201,China);Comparison of Different Extraction Methods for Total RNA from Panax notoginseng Seeds[J];西南农业学报;2013-01
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1 SHI Yun-qing1,CHEN Gao2,SUN Bing2,SHAN Shi-hua1,CHEN Jing1,MIAO Hua-rong1,HU Xiao-hui1 (1.Shandong Peanut Research Institute,Qingdao 266100,China;2.Northeast Agricultural University,Haerbin 150030,China);Cloning and Sequence Analysis of NBS Class of Resistance Gene Homolog from Peanut Testa[J];山东农业科学;2009-12
2 CHEN Gao1,2,YAN Cai-xia1,LI Chun-juan1,ZHANG Ting-ting1,SHAN Shi-hua1(1.Shandong Peanut Research Institute,Qingdao 266100,China;2.Northeast Agricultural University,Harbin 150030,China);Activity Changes of Peroxidase and Catalase in Peanut Before and After Invaded by Aspergillus Flavus[J];山东农业科学;2009-03
3 CHEN Gao1,2, XU Feng-Hua1, SHAN Shi-Hua2, ZHANG Ting-Ting2, SUN Bing1,2, LI Chun-Juan2, YAN Cai-Xia2 1.Northeast Agriculture University, Harbin 150030;2.Shandong Peanut Research Institute, Qingdao 266100;China;Research Advance of Peanut Resistance Gene Isolation Cloning[J];生物技术通讯;2009-02
4 CHUN Jun,ZHENG Yan-Feng,WANG Sheng-Hua,CHEN Fang (College of Life Science,Sichuan University,Chengdu 610064,China);A RNA Isolation Method Suitable for a Wide Range of Materials[J];生物化学与生物物理进展;2008-05
5 Zhou Zhou, Deqiang Zhang, Mengzhu Lu ① Key Laboratory of Forest Silviculture of the State Forestry Administration , Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China;Cloning and Expression Analysis of PtFATB Gene Encoding the Acyl-acyl Carrier Protein Thioesterase in Populus tomentosa Carr[J];遗传学报;2007-03
6 LIU Yi-ke~(1,2),SUN Hong-bo~2,JIAN Bo~2,HU Po~2,GAO Xiao-wei~2,HOU Wen-sheng~(1,2)(1 College of Agronomy,Northwest A & F University,Yangling,Shaanxi 712100,China;2 Crop Science Research Institute,Chinese Academy of Agricultural Sciences,Beijing 100081,China);Two efficient improved methods for isolation of high quality total RNA from soybean[J];西北农林科技大学学报(自然科学版);2006-12
7 LIU Yong-zhong LIU Qing TAO Neng-guo DENG Xiu-xin~ **(National Key Laboratory of Crop Genetic Improvement,College of Horticulture and Forestry Science,Huazhong Agricultural University,Wuhan 430070,China);Efficient Isolation of RNA from Fruit Peel and Pulp of Ripening Navel Orange(Citrus sinensis Osbeck)~*[J];华中农业大学学报;2006-03
8 Du Xihua; Lu Hai; Gao Shumin; Li Fenglan. School of Biological Science and Biotechnology, Beijing For. Univ., 100083, P. R. China.;Method for RNA isolating from anthers of Xanthoceras sorbifolia Bunge.[J];北京林业大学学报;2003-01
9 JIANG Hui-fang, WANG Sheng-yu, REN Xiao-ping ( Oil Crops Research Institute, CAAS, Wuhan 430062, China );Reaction of groundnut germplasm to Aspergillus flavus invasion[J];中国油料作物学报;2002-01
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1 PAN Jin-jin DAI Xian-hua PAN Dan-qing;Implementation ofG.729ESpeech Coding Algorithm based on TMS320VC5416 G.729Ealgorithm[J];微计算机信息;2008-14
2 CHEN XIAOLI XU JINFU;Pitch detection with the combination of wavelet transform and second spectrum[J];微计算机信息;2007-04
3 (Chongqing Technology and Business University,Chongqing 400020)Xu Su;Realize with DSP the real-time pronunciation is compressed[J];微计算机信息;2007-02
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1 SONG Yi-qing,HE Song (School of Computer and Information Engineering,Changzhou Institute of Technology,Changzhou 213002,China);A Novel Method for Transformation Between Linear Quantification and Non-linear Quantification of the Phonetic Signals Based on DSP[J];微电子学与计算机;2008-11
2 DONG Bin ZHAO Qingwei YAN Yonghong (Zhongke Xinli Speech Laboratory,Institute of Acoustics,Chinese Academy of Sciences Beijing 100080);Objective evaluation of vowels of standard Chinese pronunciation based on formant pattern[J];声学学报;2007-02
3 XIE Xue-jun~1,2,YE Yi-zheng~1,QIU Shan-qin~2,YU Ming-yan~1(1.Microelectronics Center,Harbin Institute of Technology,Harbin,Heilongjiang 150001,China; 2.Software and Integrated Circuit Promotion Center,Ministry of Information Industry,Beijing 100038,China);Data Value Prefetching Method Based on Markov Model[J];电子学报;2007-02
4 (Chongqing Technology and Business University,Chongqing 400020)Xu Su;Realize with DSP the real-time pronunciation is compressed[J];微计算机信息;2007-02
5 CHEN Zi-qiang, LIU Qing-hua (Department of Communication and Information Engineering, Guilin Institute of Electronic Technology, Guilin 541004, China);Design and Implementation of a Speech Processing Platform[J];电声技术;2006-04
6 WANG Bo, XU Wei-hang(Jiangsu Broadcasting & Television Projecting and Transmitting Station, Nanjing 210013, China);Parameters and measurement of AES/EBU digital audio[J];电声技术;2005-04
7 WANG Jin-ming, ZHANG Xiong-wei (Institute of Communication Engineering, PLA Univ. of Sci. & Tech.,Nanjing 210007, China);Study and Simulation of the Acoustic Features in Speaker Recognition System[J];系统仿真学报;2003-09
8 Wang Kunlun (Computer Science Department, Xinjiang Normal University, Uramqi, 830054 );OBTAINMENT AND CHOICE OF SIGNAL FEATURES IN PHONETIC DISTINGUISHMENT[J];新疆师范大学学报(自然科学版);2000-02
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 LI Miao;LI Guoqi;Key Lab for Restoration and Recovery of Degraded Ecosystem in Northwestern of Ministry of Education,Ningxia University;Key Lab of Agricultural Biotechnology of Ningxia;;Cloning and Sequence Analysis of Chalcone Synthase Gene(CHS)from Apocynum venetum[J];西北农业学报;2015-12
2 Zhang Qing;Liu Shuai;Xing Yu;Cao Qingqin;Qin Ling;Beijing Key Laboratory of New Technology in Agricultural Application, Beijing University of Agriculture;;Comparison of Extraction Methods of Total RNA from Strawberry Fruits[J];中国农学通报;2015-31
3 ZHANG Yuan-yuan;ZHAO Ji-qiang;JIANG Li-jun;SHI Yan-hui;CHEN Lei;GUO Shan-li;SONG Jian-cheng;Paula E Jameson;School of Life Sciences, Yantai University;School of Biological Sciences, University of Canterbury;;High Quality RNA Isolation from Mature Seeds of Maize Based on Modified and Optimized Protocols[J];玉米科学;2015-04
4 DU Yulin;WANG Weidong;WANG Yuhua;LI Xinghui;Tea Research Institute, Nanjing Agricultural University;;Subcellular Localization of CsE1α as well as Cloning and Expression of Its Promoter from the Pollen of Camellia sinensis[J];茶叶科学;2015-03
5 ZHENG Ruidong;YAO Qicong;LIAO Peng;WU Manye;Training Center,Jieyang Vocational and Technical College;Guangzhou Gene Denovo Biological Technology Co.,Ltd.;Bioengineering Department,Jieyang Vocational and Technical College;;Comparison of Different Extraction Methods of Total RNA from Panax pseudoginseng Root[J];贵州农业科学;2015-05
6 WANG Cheng-long;YUAN Chao-zheng;CHEN Hai-xia;JIANG Hui;College of Horticulture and Landscape, Hunan Agriculture University;;Detection of CMV Infecting on Longshan Lilium tigrinum[J];天津农业科学;2015-05
7 ZHAO Shanshan;GUO Yuqiong;PAN Yibin;WANG Zhong;LAI Zhongxiong;;Studies on Extraction of Total RNA from Tea(Camellia Sinensis)[J];龙岩学院学报;2015-02
8 QIAO Pu;CHEN Zi-jun;JIN Zi-an;LU Wen-cheng;SONG Yu-zhu;ZHANG Jin-yang;HAN Qin-qin;Faculty of Life Science and Technology,Kunming University of Science and Technology;;Cloning and Expression Analysis of a Gene Sucrose Transporter in Navel from Binchuan,Yunnan[J];昆明理工大学学报(自然科学版);2015-01
9 Wang Yingying;Liu Yu;Ji Yanru;Zhang Zhenghai;Zhou Guangqi;Liu Yufeng;School of Bioengineering,Dalian Polytechnic University;Daqing Branch of Heilongjiang Academy of Sciences;;Cloning of DGAT1 Gene from Perilla frutescens and Construction of Scenedesmus quadricanda Expression Vector[J];生物技术通报;2014-05
10 JIANG Hui;YUAN Chao-zheng;CHEN Hai-xia;College of Horticulture and Landscape, Hunan Agriculture University;;Detection of LSV Infecting Longshan Lilium tigrinum[J];天津农业科学;2014-05
【Secondary Citations】
Chinese Journal Full-text Database 1 Hits
1 CHEN Qing-hong,ZHAO Zhi-jin,WU Jie (Hangzhou Institute of Electronic Engineering Hthe School of Communication, Hangzhou Zhejiang 310018 China);Research on Estimation of Pitch Period OF MBE Vocoder[J];杭州电子工业学院学报;2004-01
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