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《Chinese Agricultural Science Bulletin》 2011-01
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The Comparison of Different Methods for Isolating Total RNA from Peanuts

Chen Gao 1,2 , Shan Lei 2 , Zhou Lixia 1,2 , Tang Guiying 2 , Bi Yuping 1,2 ( 1 Science College, Shandong Normal Unveristy, Jinan 250014; 2 High-Tech Research Center, Shandong Academy of Agricultural Science, Key Laboratory for Genetic Improvement of Crop, Animal and Poultry of Shandong Province, Key Laboratory of Crop Genetic Improvement and Biotechnology, Huanghuaihai, Ministry of Agriculture, Jinan 250100)  
In order to obtain better quality total RNA in different parts of peanuts, two RNA extraction methods (CTAB and Trizol) and two RNA extraction kits (AXYGEN and TIANDZ) were compared. The results showed that the improved CTAB method was better than Trizol method and two total RNA extraction kits. The total RNA isolated with the improved CTAB method had high integrity, high yield, good stability and no DNA pollution, and then could be used for semi-quantitative RT-PCR and so on.
【Fund】: 山东省自然科学基金项目“花生酰基载体蛋白基因的克隆与功能分析”(ZR2009DM013);; 国家自然科学基金项目“花生二酰甘油酰基转移酶DGAT基因多态性及其对种子油份含量和油份品质的影响”(30871541)
【CateGory Index】: S565.2
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