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《Chinese Agricultural Science Bulletin》 2011-07
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Correlative Analysis Between Polymorphism of PGC-1α Gene and Meat Quality Traits in Qingyuan Partridge Chickens

Liu Hongxiang 1,2,Shu Jingting 2,Li Huifang 2,Chen Kuanwei 2,Wang Jinyu 1 (1 College of Animal Science and Technology,Yangzhou University,Yangzhou Jiangsu 225009;2 Jiangsu Institute of Poultry Science,Yangzhou Jiangsu 225003)  
The PGC-1α gene is the key gene impacting meat quality.The polymorphism of PGC-1α gene was studied in order to find out some mutation sites having more influences on meat quality,which can provide the foundation of next functional verification of protein.The polymorphism sites of PGC-1α gene of qingyuan partridge chickens was found by PCR-SSCP and PCR-LDR.Fat content,shear force and WLR (water lost rate of breast muscle ) tested as meat quality indicators were grouped according to different genotypes.And then analyzing the impact of different genotype on meat quality via analysis of variance and multiple comparison.Two polymorphism sites SNP1 and SNP2 were screened in qingyuan partridge chickens which were both mutated from G to A.SNP1 was in an intron,which had no effect on meat quality.SNP2 was a sense mutation in the exon 8 with a significant effect on WHC (water holding capacity).Hens ' meat quality was better than cocks ' ,and there was extremely significant difference between the two sexes as to the shear forces.As for SNP2,the meat quality of AG genotype was better than of AA and GG genotypes.
【Fund】: 现代农业产业技术体系建设专项资金(nycytx-42-G1);; 江苏省自然科学金基金(BK2009177);; 江苏省家禽科学研究所青年(博士)基金(JQ2009001)
【CateGory Index】: S831.2
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