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《Chinese Agricultural Science Bulletin》 2012-11
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Different Generations of Bovine Mammary Epithelial Cells Impacting on Gene Expression of Milk Protein

Han Yanan 1,Hou Xianzhi 1,Kao Guilan 1,Wang Xiumei 1,Yang Jinli 1,Gao Aiwu 2,Yang Yinfen 1,Zhao Qing 1,Gao Min 3 (1 College of Animal Science and Veterinary Medicine,Hohhot 010018;2 College of Food Science and Engineering,Inner Mongolia Agriculture University,Hohhot 010018 ;3 InnerMongolia Academy of Agriculture and Animal Husbandry,Hohhot 010010)  
The aim of this study was to research milk protein gene expression—αs1-casein,β-casein,κ-casein of lactating bovine mammary epithelial cells between different generations;Using the real-time PCR method to detect milk protein gene expression of lactating bovine mammary tissue,the cells of primary generation (P0) derived from the bovine mammary tissue,the first generation (P1),the second generation (P2),the third generation (P3),the fourth generation (P4) and the fifth generation (P5);The results showed that expression of three target-genes was at different levels between the cells of primary generation and passages.The expression in the primary cells was significantly higher than P1-P5 cells (P0.05),whereas P1-P5 cells had no significant difference (P0.05).Moreover,from P 0 to P 5,the expression of αs1-casein,β-casein,κ-casein was gradually decreasing.So,P2-P5 BMECs could be used as a trial material.
【Fund】: 现代农业产业技术体系建设专项资金资助;; 内蒙古自然基金项目“奶牛乳腺上皮细胞三维培养体系的建立及其对酪蛋白合成的影响”(2010MS0406);; 国家“973”项目“牛奶重要营养品质形成与调控机理研究”(2011CB100803)
【CateGory Index】: S823.91
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