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《Chinese Agricultural Science Bulletin》 2012-33
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Identification and Antagonistic Activity of Bacillus subtilis E-1

Wang Qianlong 1,Chen Xinchun 1,Peng Shaodan 1,Xie chunqiong 2,Ji Guanghai 3,Wei Lanfang 1(1 Teaching Center of the Basic Experiments of Agricultural Majors,Yunnan Agricultural University,Kunming 650201;2 College of Resources and Environment,Yunnan Agricultural,Universtiy Kunming 650201 ;3 College of Plant Protection,Yunnan Agricultural,Universtiy Kunming 650201)  
Antagonistic strain E-1of rhizobacteria against the fungi and bacterial pathogen were selected on NA and potato dextrose agar with many rhizobacteria isolated from the rhizosphere soil of Ageratina adenophora Sprengel from Kunming suburbs in Yunnan,respectively.Identification on basis of phenotypic and physiological and biochemical properties from biolog data indicating that strain E-1 homologized with which descripted for genus Bacillus.16s rDNA determination and analysis was used for further identification,which showed that 16s rDNA sequence of strain E-1 shared 100% homologies with published sequence of Bacillus subtilis GZB from GenBank,and belonged to the same branch in phylogenetic tree.In vitro antagonistic assay showed strain E-1 could suppress the growth of five plant bacterial and five plant fungal pathogens.The results showed that strain E-1 had different level antagonistic activity to the fungi and bacterial pathogen.And maybe potential used for biological control of plant diseases.
【Fund】: 云南农业大学农科专业基础实验教学中心大学生创新基金项目“紫茎泽兰根际微生物和内生细菌的分离鉴定及应用”(NKZX2011CX027);; 云南省第十一批学术技术带头人后备人才基金项目“云南省重要细菌病害研究”(2008PY042)
【CateGory Index】: S476
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