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《Chinese Agricultural Science Bulletin》 2012-34
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Screen and Identification of High Copper-resisting Fungi and Its’Application in Absorbing Copper from Tea Garden Soil

Fu Donghe 1,2,Xue Zhihui 1,Yao Yali 1,Zeng Tingyu 1,Lu Xiangyang 2(1 National Research Center of Engineering Technology for Utilization of Functional Ingredients from Botanicals,Changsha 410128;2 College of Life Science and Technology,Hunan Agricultural University,Changsha 410128)  
The aims were to screen and identify a microorganism strain with absorbing and resisting to copper and to use it removing heavy metal copper in the environment.By using microorganism screening and separating methods,the copper-resisting microorganism were widely obtained from the soil around pig farms with a long captive time.The target microorganism strains were screened and purified repeatedly.Their morphology and molecular identification were analyzed and the microorganism strains were applied to absorb heavy metal copper in tea garden soil.Morphological characteristics were observed under the 400 times optical microscope and scanning electron microscope.Amplification of DNA sequencing fragment based on the 5.8S rDNA universal primers were compared in the GenBank database using the BLAST tool.10 strains of fungus of different copper-resisting concentrations were separated.The highest concentration of copper-resisting strain of the fungus was A 4 which concentration of copper-resisting reached 940mg/L.A 4 bacteria grew fast in Czapek medium.It was identified synthetically as Aspergillus fumigatus.The strain A 4 had good absorption to copper in tea garden soil.
【Fund】: 湖南农业大学博士后基金“富铜富锌微生物的筛选及在环境重金属处理中的应用”(71709)
【CateGory Index】: X172
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【Citations】
Chinese Journal Full-text Database 5 Hits
1 Cao Xiao-hong et al;Study on Screening of Tolerant-Lead & Copper Strains and Its Adsorption Capability[J];环境保护科学;2008-05
2 LIU Hong-Tao,ZHENG Guo-Di,CHEN Tong-Bin,GAO Ding,SONG Bo,YANG Jun,CAI HongCenter for Environmental Remediation,Institute of Geographic Sciences and Natural Resources Research,Chinese Academy of Sciences,Beijing 100101,China;Major input of copper to farmlands and control for its pollution risk[J];生态学报;2008-04
3 WANG Jin,ZHANG Qing-qing,TANG Bin(Dept.of Bioch.Engn.,Anhui University of Technology and Science,Wuhu 241000,China);Isolation of a high anti-Cu~(2+) strain and study on its bioaccumulation characterization[J];安徽工程科技学院学报(自然科学版);2007-01
4 ZHAO Qing-liang,ZHANG Jin-na,LIU Zhi-gang,YOU Shi-jie,WANG Shao-hua,WANG Li-na,XUE Shuang(School of Municipal and Environmental Engineering,Harbin Institute of Technology,Harbin 150090,China);Effect of Reclaimed Water Used for Irrigation on the Quality of Crops and Soil[J];环境科学;2007-02
5 Zhang Zhihua Hong Kui (Institute of Tropical Biological Sciences, CATAS, Haikou, Hainan, 571101);Application of Nucleic ACID Sequence Analysis in Fungi Taxonmy[J];华南热带农业大学学报;2006-02
【Secondary Citations】
Chinese Journal Full-text Database 1 Hits
1 LIU Xiao rong 1, QIU Guan zhou 2, HU Yue hua 2, YANG Jun he 1, JIN Ming lin 1 (1. Department of Materials, Shanghai Institute of Technology, Shanghai 200233, China; 2. School of Resource Processing and Bioengineering, Central South University, Changsha 410083, China);Effect of Lix984N content on phase disengagement dynamics in copper-SX[J];Transactions of Nonferrous Metals Society of China;2003-04
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