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Study on Physiological Activity and Pollination Characteristic of Stigma and Pollen of'Dangshan suli'

Guo Yuan 1,2,Shao Youquan 1,Ma Weihua 1,Shen Jinshan 1,Guo Baobei 1,Wu Wenqing 1(1 Institution of Horticulture,Shanxi Academy of Agricultural Sciences,Taiyuan 030031;2 College of Life Science,Shanxi University,Taiyuan 030006)  
Using the semi-vitro culture and benzidine-H2O2 methods to study the pollen germination characteristic and stigma receptivity of ' Dangshan suli ',through the research of optimal period of pollination and the fruit set percentage of seven pollination treatments to study pollination characteristic of pear.The results showed that:the optimal time of stigma activity was at 11:00-15:00 in each day after flowering,the optimal period of pollination of the stigma was the 4th to 5th day after flowering.The optimal temperatures of pollen germination and growth of pollen tube were 25℃ and 22-25℃ respectively,the percentage of pollen germination and the length of pollen tube would be increased as time increase at the same temperature.Fruit set percentage of flowers of seven pollination treatments,the fruit set percentage without insects ' pollinating was 0.48%-0.44%,fruit set percentage with natural pollination and artificial pollination were 2.83% and 13.05% respectively,fruit-set percentage with bees ' pollination for treatment of ' Dangshan suli ' versus ' Xuehuali ' was 32.95%,and fruit set percentage of the fresh pollen and commodity pollen was 70.2% and 13.05% respectively.It was showed that ' Dangshan suli ' was entomophilous and cross-pollinating fruit tree,and bees could pollinate for ' Dangshan suli ' to improve the fruit set percentage of it when the stigma activity was in the highest period.Through the research of physiological activity of floral organ and fruit set habit,it also could provide the basic information of pollination and production for ' Dangshan suli '.
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