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《Chinese Agricultural Science Bulletin》 2014-13
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In Vitro Pollen Germination Study on A Rare and Endangered Specie: Pinusdabeshanensis Cheng et Law

Han Jianwei;Wang Enmao;Zhang Zhiyong;Li Yingyue;Key National Engineering Laboratory for Tree Breeding/ Key Laboratory for Genetics and Breeding of Forest Trees and Ornamental Plants, Ministry of Education, Beijing Forestry University;Forestry Center in Yuexi County;  
In order to detect the reasons why the Pinusdabeshanensis Cheng et Law is endangered and providethe evidence for the breeding and conservation. The author applied designed the orthogonal test to identify theeffect of sucrose, boron, calcium on germination rate and pollen tube length using in vitro pollen germinationmethods. The results showed that: sucrose was the key factor in its germination and growth followed by boricacid, and calcium chloride played the least role. The ratio of pollen germination could be significantlyincreased by adding sucrose while it also could be limited when the sucrose was in the high level. Appropriateaddition of boric acid could promote pollen germination rather than pollen tube growth. Calcium chloride hadlimited effect on pollen germination and it seemed that germination rate was not sensitive to the level ofcalcium chloride. Integrating pollen germination rate and tube growth, the optimum medium forPinusdabeshanensis Cheng et Law pollen culture should be 4% sucrose+0.05% boric acid+0.025% calciumchloride.
【Fund】: 国家林业局珍稀濒危野生动植物救护与繁育项目“大别山五针松种子发育与遗传多样性保护研究”
【CateGory Index】: S791.24
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