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《Chinese Agricultural Science Bulletin》 2015-04
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Germination Characteristic and Endogenous ABA, IAA Content Change with HPLC in the Seed of Hedysarum laeve Maxim During Stratifying, Shelling

Niu Yajie;Yuan Jinfeng;Chen Hongxian;Dong Qianqian;Fan Yali;Wang Xiaofeng;Liu Zhonghua;College of Biological Science and Biotechnology, Beijing Forestry University;  
In order to determine the internal factors inhibited seed germination of Hedysarum laeve Maxim, themoisture, absorbent law, seed germination characteristics, endogenous ABA and IAA content of shelled or podsunder cold stratification were studied. The results showed that the moisture of seeds with pods was 9.16%,while the shelled was 8.92%; the soaking process all included sharp soaking, stable soaking and saturatedsoaking stages. The maximum water absorption rate of the shelled reached 0.7475 g/h, which was 4.79 times ofthe pods, and the shelled spent about half time reaching saturated soaking stages compared with the pods. Theshelled germination rate and germination index could attain 81.67% and 33.27, which were 8.167 and 38.39 times contrasted with the control group, pods with 60-day cold stratification was following, which reached46.67% and 4.19; the inhibitor ABA content of CK was relatively the highest, reaching 7921.12 ng/m L, ABAcontent after 80-day cold stratification could drop down to 4590.85 ng/m L. If the germination percentage,germination index, endogenous ABA content and IAA/ABA ratio were all considered, it is suggested that theoptimum condition to improve seed germination characteristics for Hedysarum laeve Maxim should be shelled to improve their water absorption or the pods treated by cold stratification at 4℃, 60-day.
【Fund】: 林业公益性行业科研专项经费项目“种子超干及包覆技术在沙区生态修复中的应用研究”(201104024)
【CateGory Index】: Q945.34
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