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《Scientia Agricultura Sinica》 1982-02
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STUDY ON TISSUE CULTURE AND ITS APPLICATION FOR THE RAPID PROPAGATION OF RAMIE

Yan Changjing Zhao Qinghua (Institute of Crop Breeding and Cultivation, Shanghai Academy of Agricultural Sciences, Municipality of Shanghai) Hu Jijin Zhou Puhua Fan Hongzhi Li Zongdao Peng Chongli Wang Chuntao (Hunan Agricultural College, Changsha, Hunan Province)  
Shoot formation was successfully differentiated from calli of the stem, leaf and hypocotyl of ramie (Boehmeria nivea L.)in the MS+6-BA 2-3mg/1 medium, and the axillary buds developed from these shoots could grow normally. When these shoots were transplanted to (1/2) MS+NAA 0.2 mg/1 medium, they regenerated into complete plantlets which could grow up normally in the field. We used the method of axillary buds for rapid propagation, and it could produce a greater number of tube plantlets free from morphological variation within a short period. It could satisfactorily meet the need for the propagation of fine ramie varieties and the acceleration of breeding programmes. Besides, the influence of different plant hormones on root growth was studied. In the (1/2)MS +IAA0.1ppm+NAA0.2ppm medium, the roots could grow more prosperously andstrongly. A whole series of transplanting technique had been obtained.
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