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《Scientia Agricultura Sinica》 2001-06
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Effects of Cool Pretreatment on LeveL of Amino Acid and Polyamine Content in Barley Anthers

HUANG Jian-hua, LU Rui-ju, WANG Yi-fei, SUN Yue-fang, ZHOU Run-mei (Biotech. Research Center, Shanghai Academy of Agricultural Sciences, Shanghai 201106) (Cytogenetics Institute, Nanjing Agricultural University, Nanjing 210095)  
Using barley lines with low and high responding to anther culture as materials , changes of endogenous amino acid and polyamine contents in anthers were investigated. Cool-pretreatment of panicles for 5 days led to an decrease of total free amino acid content and 15 days of the pretreatment led to a big in- crease of the total content. In comparison with low responding lines, the total content of high responding line increased more obviously, especially for proline, serine, arginine and alanine. After 25 days of cool- pretreatment, the total content of polyamine was significantly higher than that of 0 day's treatment in high responding line and decreased in low responding lines. The contents of putrescine and spermidine increased more obviously in high responding line and decreased significantly in low responding lines. It can thus be suggested that large quantity of free amino acid decomposed from anther-protein and increase of free pro- line, serine, arginine, alanine and putrescine, spermidine benefits the microspores in starting its first mi- totic division.
【Fund】: 国家“九五”科技攻关项目资助(96-C01-01-02-14)
【CateGory Index】: S512.3
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