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Application of Near Infrared Transmittance Spectroscopy (NITS) to the Determination of Amylose Content of Rice Grain

XIAO Xin1,XIE Xin-hua1,2,CHEN Yi1,LUO Weng-yong1,CHEN Jian-wei1,LIU Yan-zhuo1,MAO Xing-xue1,LI Xiao-fang1(1 Key Lab of Rice Genetics and Improvement,Ministry of Agriculture of China /Guangdong Key Lab of New Technology in Rice Breeding,Rice Research Institute of Guangdong Academy of Agricultural Sciences,Guangzhou 510640;College of Food and 2 Engineering,Northwest Sci-Tech University of Agriculture Forestry,Yangling 712100)  
One hundred and twenty-four samples of small amount of rice grain were scanned by Foss Tecator Infratec 1255, and their amylose contents were determined by chemistry method. With the WINISI calibration maker software, several mathematics treatments to the sample spectra and several different regression methods were adopted to optimize a good calibration equation for determination of amylose content in small amount of rice grain. The standard error of calibration (SEC), standard error of cross-validation (SECV) and regression squared (RSQ) of the calibration selected were 1.489, 1.761 and 0.885.
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