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《Scientia Agricultura Sinica》 2005-07
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Construction and Analysis of Soybean Genetic Map Using Recombinant Inbred Line of Charleston × Dongnong 594

CHEN Qing-shan1, ZHANG Zhong-chen1, LIU Chun-yan1, WANG Wei-quan2, LI Wen-bin1 (1Soybean Research Institute, Northeast Agricultural University, Harbin 150030; 2Sun Yat-sen University, Guangzhou 510275)  
F2:10 RIL population with 154 lines, crossed by Charleston as female parent and Dongnong 594 as male parent were used in this experiment. One hundred and sixty-four SSR primers were screened with the two parents and amplified on the 154 lines. A new soybean molecular genetic map, named NEAUSRI-GMS, was constructed by Mapmaker. The total length of the soybean genetic map is 1913.5 cM, and the average distance among markers is 11.89 cM. The length of linkage group varied from 0.4 cM to 309.5 cM, and the markers on the linkage group varied from 2 to 28. The distribution of SSR markers on every linkage group is not even. High density region of markers existed on linkage group A1, C2, and D1a. Compared with 5 soybean genetic maps constructed at home and abroad, NEAUSRI-GMS has high homologous with the public genetic map abroad.
【Fund】: 国家“863”计划(2001AA211041);; 哈尔滨市青年科学研究基金(2002AFQXJ043);; 国家科技攻关项目(东北区2004BA907A26)
【CateGory Index】: S565.1
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