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《Scientia Agricultura Sinica》 2006-03
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Development of Amplified Consensus Genetic Markers in Gramineae Based on Rice Intron Length Polymorphisms

LU Yong-quan1,2, WANG Xu-sheng1, HUANG Wei-su1, XIAO Tian-xia1, ZHENG Yan1, WU Wei-ren1,3 (1College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029; 2Biotechnology Research Center, Heilongjiang Academy of Agricultural Sciences, Haerbin 150086; 3College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou 350002)  
By designing primers in the conservative coding sequences flanking a polymorphic site, it is possible to exploit a PCR-based marker called amplified consensus genetic marker (ACGM), which is transferable among different species.【Objective】The purpose of this study was to investigate the feasibility of developing ACGMs in Gramineae using the published genomic sequences of indica and japonica rice【.Method】Based on the intron length polymorphisms between the two rice subspecies, 38 pairs of ACGM primers were developed. Twelve accessions representing 6 grass genus including Oryza, Zea, Setaria, Triticum, Phyllostachys, Echinochloa crusgalli Beauv and Spartina anglica were used to verify the candidate ACGM markers【.Result】Almost all primers could acquire specifically amplified products in at least one accession. More than 1/3 primers could obtain specifically amplified products in all accessions. For each accession, about 2/3 primers on average could obtain specifically amplified products. The proportion of polymorphisms between species or subspecies within genus was 24.1%-90.3%, with an average of 44.6%.【Conclusion】These results showed that developing ACGM in Gramineae using rice genome data is feasible.
【Fund】: 国家“863”计划项目(2003AA207160);; 福建省重大科技专项(2004NZ01-2)
【CateGory Index】: S511
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