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《Scientia Agricultura Sinica》 2013-19
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Identification of Viruses from Potato Planted in Winter in Guangxi by Small RNA Deep Sequencing

SONG Jing-jing;MENG Jiao-rong;ZOU Cheng-wu;LI Pu;WANG Zhi-qiang;CHEN Bao-shan;College of Agriculture,Guangxi University;State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources,Guangxi University;College of Life Science and Technology,Guangxi University;  
【Objective】The objective of this study is to identify viruses infecting potato planted in winter in Guangxi for establishing a future healthy potato seed program and better disease control in the field. 【Method】One hundred and nine samples with viral disease-like symptoms were collected from potato plants in the fields in 2012 and subject to initial ELISA screening before they were pooled and analyzed by small RNA deep sequencing. Viruses identified by small RNA sequencing were validated by virus-specific RT-PCR from selected groups of samples.【Result】Potato leafroll virus(PLRV), Potato virus A(PVA), Potato virus H(PVH), Potato virus M(PVM), Potato virus S(PVS), Potato virus Y(PVY), and Potato spindle tuber viroid(PSTVd) were detected in the samples and PVS was found to have a rich genetic diversity.【Conclusion】Potato planted in winter in Guangxi were infected with many viruses including two viruses and a viroid that had not been reported in the region before. Thus, a virus-free healthy potato seed program is needed in urgency.
【Fund】: 国家重点基础研究“973”计划项目(2006CB101900)
【CateGory Index】: S435.32
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