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《Central South Pharmacy》 2007-06
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HPLC Fingerprint of Potentilla anserine anserina

HOU Lu-xing1,2,CAI Guang-ming1,ZHANG Ya-ming1,HUANG Yuan3,ZHANG Zhuo-yong2(1.Institute of Chinese Materia Medica,No.302 Hospital of the PLA,Beijing 100039;2.Department of Chemistry,Capital Normal University,Beijing 100037;3.Shenyang Pharmaceutical University,Shenyang 110016)  
Objective To establish the method of fingerprint analysis of Potentilla anserina by HPLC.Methods Kromasil C18(4.6 mm × 250 mm,5 μm)column was used.The mobile phase was composed of acetonitrile and water with a linear gradient elution.The flow rate was 1.0 mL·min-1 with DAD detected at 208 nm..The column temperature was 30 ℃.Results Nine peaks were gained on the HPLC fingerprint.The RSDs of relative retention time and area of common peaks which existed in all samples were less than 3%.The results were in accordance with the request of fingerprinting.Conclusion This method is accurate and reliable.The fingerprint can be used for the identification and quality control of Potentilla anserina.
【Fund】: “十五”国家科技攻关计划“西部开发科技行动”重大项目 No.2004BA901A250
【CateGory Index】: R29
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